In the Rhode River estuarine/watershed area, increased acidity of precipitation from atmospheric acidic deposition has deleterious effects on the freshwater ecosystems. One of the characteristics of an acidic watershed is the mobilization of aluminum from soils to aquatic environment. Increased concentrations of aluminum in surface waters are toxic to living organisms. Detection of long-term changes of acidity and elevated Al concentrations in surface and soil waters is a central issue. In this present paper, the dynamics ofAl speciation in the shallow ground waters from Rhode River watershed 110 (la) were investigated. This research provides a unique, regional analysis of important controls on the transport of Al speciation through the rural watershed of the Chesapeake Bay.