Bioaccumulation kinetics of 9 cholorophenols in semi-permeable membrane biomimetic sampling device and in fish was studied under controlled laboratory flowing-through conditions.Based on the difference between bioconcentration process in SPMD and bioaccumulation process of chlorophenols in fish the degradation and the structure/effects relationship of degradation of chlorophenols in fish was examined.The results showed that the chlorophenols under testing could be concentrated by SPMD in a similar way as it was concentrated by fish lipid.The biodegradability of chlorophenols decreased with increase of substitutions of chlorine atoms and is closely related to the position of chlorine substitution in the ring of benzene.