Using water and sediment of Lake Nanhu, Wuchang of Wuhan City, we designed a group of pot experiments including a control and a treatment that added 100mg/kg phosphorus to the sediment. Submerged macrophytes Potamogeton crispus, Elodea nattallii were planted in the sediment on September 15,2007. After 160 days observation, the concentration of the total soluble phosphorus(TDP) in the overlying water responded to the sediment with high phosphorus content and reduced obviously. The treat-menu all caused total phosphorus(TP),total nitrogen (TN ),and content of chlorophyll-a(Chl.a) in the overlying water obviously reduced. The effect of E. nuttallii on the total phosphorus was better than that of P. crispus, and the submerged macrophytes on controlling Chl. a of the overlying water in high phosphorus of the sediments were more efficient than those in the low phosphorus.In the submerged macrophytes cultivation experiment, whether or not applying phosphorus to the sediment the change of Chl. a a-long with cultivated time of the plants could be fitted better with the first order kinetic equation. TDP and TP of overlying water were significantly correlated with Ca-P of sediment, and the correlation coeffcient of TDP of overlying water with Ca₁₀-P of sedi-ment under growth of P. crispus and E. muttallii was 0.990 ( P≤O. 0l)and 0.977 (P≤0.05).