Sediment re-suspension is one of the important characteristics of hydrodynamic processes in shallow lakes. This experimental study was compared with the changes of zooplankton community in different levels of the re-suspension to investigate the effect of sediment re-suspension on zooplankton. The result shows significantly difference when using different treatments of concentrations of TSS and Chl. a. The cladoceran Bosmina coregona, Ceriodaphnia cornuaa and Rotifere.dominant in re-suspended groups, but Daphnia sp. is dominant in no re-suspended group. Re-suspension produced an effect of deducing size of the composition of zooplankton communities. The abundances of Daphnia carinaza, Bosmina coregoni and Rotifers are significant different in different re-suspended levels,but Ceriodaphnia wrnuta and Cabmoidas show no significant different. The concentration of TSS is significant positive correlation with abundances of Rotifers and Bosmina coregoni, but significant and negative correlation with Daphnia sp. abundance,while there is correlation with other populations. The research shows that the sediment re-suspension is one of the important processes determining zooplankton community structure.