Sediment resuspension resulted from wave actions is one of the major characters of subtropical shallow lakes. Resuspension can directly alter the zooplankton community structures, and indirectly influence the predation of planktivorous fishes. Our study aimed to reveal how planktivorous fish Hemiculter leucisculus affected the zooplankton community structures in different levels of sediment resuspension. The results showed that in the condition of H. leucisculus, large-sized cladoceran Daphnia obtusa was only found and became dominant in the treatment of weak resuspension. Biomass of D. obtusa decreased with the increase of resuspension intensity in treatments without H. leucisculus and Chydorus sp.. The Ceriodaphnia cornuta dominated the cladoceran communities in the treatments of strong resuspension without H. leucisculus or no resuspension with H. leucisculus. Regardless of fish and resuspension intensity, Mesocyclops sp. was dominant copepod and its biomass increased with the decrease of the resupension intensity. Rotifer abundances were lower than cladoceran and copepod in all treatments. This study indicates that in subtropical shallow lakes, a certain level of sediment resuspension can weaken both the predation of planktivorous fishes and resuspension effect on large-sized cladoceran, which can decrease the chlorophyll-a concentration through increasing the feeding of large-sized cladoceran on algae.