Studying on yeast diversity in plateau lakes can provide theoretical basis for protection of lake ecosystem and lay foundation for exploitation of yeast resouses.A total of 553 yeast isolates were obtained from Lake Fuxian in Yunnan Province. Compared yeast diversity indices in different regions by SPSS statistics 19.0, and spatial dynamics of yeast community and its relationship to environmental factors were evaluated.The results showed that yeasts were identified as belonging to 22 genera and 52 species and 1 presumed new species by 26S rDNA D1/D2 domain sequence analysis.Duncan test showed that Pielou evenness index of yeasts existed in southern region was higher than that of Northern region distinctly.Difference analysis revealed that content of total organic carbon in Northern district was more than that in Southern district, besides, Pearson correlation analysis displayed that content of total organic carbon was significantly positive related to yeast abundance. Redundancy analysis revealed that yeast community in Lake Fuxian was obviously in relation to content of total organic carbon. The study showed that yeast resourses in Lake Fuxian were abundant,in addition,human activities had influence on spatial dynamics of yeast community there.