Based on the laboratory simulates a static lake system, the removal of organic pollutants in sediments using zero-valent iron (Fe⁰) for in-situ sediment treatment was studied. The effects of zero valent iron (Fe⁰) on the overlying water including DO, Eh, pH and COD_Cr were investigated and the effects on the sediments including the number and kinds of organic pollutants, total organic content were discussed. The results showed that with adding Fe⁰ for treating 80 days, ① the amount of low-molecular-weight organics (of below 200) were increasing apparently which could be easy to degrade detecting by GC-MS. The removal rate of organic content in sediments was around 44%. ② the value of pH was rising to 8.4, while the Eh was declining from 150 mV to 74 mV and the DO concentration of overlying water depleted dramatically from 6.6 mg/L to 0.2 mg/L. The system formed an anaerobic environment. COD_Cr concentration of overlying water was lower than that in pure bottom-water system. In addition, there were certain relations among DO, Eh, pH and COD_Cr in overlying water. In conclusion, the removal of organic pollutants was effective by adding Fe⁰ in the sediments, and this effect on overlying water quality was not permanent and seriously.