Zoobenthos play crucial roles in maintaining and stabilizing the structure and function of lake ecosystem. Moreover, various zoobenthic species are valuable in freshwater eco-restoration. Uncovering the succession of zoobenthic community evolution could provide important information for the application of zoobenthic species in freshwater eco-restoration. In the present study, succession of macrozoobenthic communities was examined by combing field investigation from 2007 to 2017 and various documents. It was found that the succession of zoobenthos community in Lake Wuli can be divided into four stages, including natural succession stage, rapid degradation stage, habitat restoration stage and slow recovery stage. Besides, taking the water quality, sediment characteristics, phytoplankton community, and the reported eco-restoration activities in Lake Wuli into consideration, we discussed the major impact factors in different succession stages of zoobenthos community. Summarily, based on the above results and practical application of zoobenthos in freshwater eco-restoration, the present study pointed out the research directions which should be enhanced at last.