Based on the flow velocity and water quality monitoring data in three periods: flood season, dry season and normal season from 29 river network monitoring stations in Wuxi Binhu region, which is located in eastern part of Meiliang Bay, Lake Taihu in China, the river network can be divided into five regions. After analyzing the spatial and temporal heterogeneity of flow velocity and the typical water quality indexes and combining the principal component and correlation analysis, the current situation and causes of different regional hydrodynamic and water quality can be understood. The results show that the water quality and hydrodynamic conditions in Liangxi River and Caowangjing River are good, and most of the water quality factors show strong correlation with the flow rate. The regional variation of water quality and flow velocity in Mali Port is obvious, showing weak correlation. In the north and south of the urban river network, the flow rate is slow, the river pollution load is heavy, and the correlation between the flow rate and water quality factors is low. Through the field monitoring of flow velocity and water quality in the Binhu River network, the actual effects of flow velocity on the water environment of the river network can be analyzed, and the response relationship between different water quality indexes and flow velocity have been verified, which provides basic support for the water quality protection and scientific water pollution treatment technology in the Binhu River network area. Thus, it is of great significance to improve the water ecological environment.