A confirmatory study on the distribution of anadromous Coilia nasus was conducted using otolith strontium and calcium microchemistry, based on 21 fishes sampled at the Luziyao section of the Ganjiang River in Lake Poyang Basin on May 27th, 2020. The results indicated that 16 individuals were anadromous-long supermaxilla ecomorphotype and constituted as high as 76% of the total sampled fish. The proportion is far higher than those reported in literature till now for C. nasus around the Lake Poyang and its tributaries. The spawning site of the fish possibly exists in the Gangjiang River. The present study firstly confirmed the existence of anadromous C. nasus in the Ganjiang River and provided objective evidence on the recovery tendency of resource and habitat for this fish under the national policies of ecological protection and the corresponding ban on fishing (started on February 1st, 2019) in the Yangtze River. To rescue protection of resources and key habitats for C. nasus in the Gangjiang River, further studies are urgently needed to more accurately locate the distributing areas and spawning sites, and assess the potential impacts from human activities (e.g., hydraulic engineering, sand mining of river bed) to C. nasus.