To investigate the patterns and determinants of zooplankton community stability in different regions of Lake Dianchi, seasonal surveys were conducted in Caohai, Dabokou and Waihai in 2020. The results showed that dissolved oxygen and transparency were significantly higher in Dabokou than in Waihai. Meanwhile, the concentrations of total nitrogen, total phosphorus, suspended solids, chlorophyll-a and chemical oxygen demand were significantly lower in Dabokou than in Waihai, and were significantly different from Caohai compared to both Dabokou and Waihai. A total of 41 genera of zooplankton (12 genera of cladocerans, 8 genera of copepods and 21 genera of rotifers) were identified in the three regions. Both species number and density of rotifers were dominant during the surveys. The annual density of zooplankton in Dabokou (7771.3 ind./L) was higher than in Caohai (2901.1 ind./L) and Waihai (634.8 ind./L), while the annual mean biomass of zooplankton in Caohai (3.72 mg/L) was higher than in Dabokou (2.15 mg/L) and Waihai (2.09 mg/L). The results of non-parametric multiple analysis of variance and similarity percentage analysis showed that there were significant differences between the zooplankton communities in the three regions. The community structure of Dabokou (ecological restoration region) was significantly different from that of Caohai and Waihai, and the determinant of the difference was rotifer species. The community structure of cladocerans and rotifers was significantly different between Caohai and Waihai. The stability of the zooplankton community in Waihai was significantly higher than that in Caohai and Dabokou, which was significantly positively correlated with species diversity. Pearson correlation analysis and redundancy analysis showed that the driving environmental factors of zooplankton community stability were dissolved oxygen, pH and chlorophyll-a. The study implied that improving the water environment could reduce the stability of zooplankton community dominated by pollution olerant species.