Metabolites such as 2-methylisoborneol (2-MIB), produced by filamentous cyanobacteria in Taihu Lake, pose a significant threat to the safety of drinking water sources. However, research on the spatiotemporal variation of 2-MIB concentrations in Taihu Lake remains limited. To elucidate the spatiotemporal dynamics and influencing factors of 2-MIB in key source water areas of Taihu Lake, this study focused on Eastern Taihu Lake. Monthly monitoring during the high-temperature period of 2023 and intensive monitoring in critical zones were conducted to analyze the succession of major odor-producing cyanobacteria, the spatiotemporal characteristics of 2-MIB, and its primary driving factors.Monthly investigations revealed that Planktothricoides sp. was the dominant odor-producing cyanobacterium in Eastern Taihu Lake. However, compared to 2022, the peak relative abundance of the mic gene in Planktothricoides sp. decreased from 94.4% in 2022 to 90.8% in 2023. The highest lake-wide 2-MIB concentration (306.3 ng/L) occurred in July. Similarly, the peak concentration (507.8 ng/L) in the Taipu River Estuary - Dongjiaozi area was also observed in July. The highest concentration (352.0 ng/L) in the Taipu River Estuary - Guajingkou area occurred in August.Intensive monitoring in critical zones identified July 13th as the date with the highest recorded 2-MIB concentration (769.6 ng/L).Concentrations in the Taipu River Estuary exhibited dramatic fluctuations, reaching a maximum of 2185.0 ng/L, significantly higher than concentrations observed at Guajingkou or the inflow area from the main body of Taihu Lake.Correlation analysis indicated that water temperature is a prerequisite condition for high 2-MIB events. Total phosphorus (TP) showed a significant positive correlation with 2-MIB concentration, while ammonia nitrogen (NH?-N) exhibited a significant negative correlation. Further analysis of wind speed in the 24 hours preceding high 2-MIB sampling events revealed that wind speeds exceeding 3 m/s caused short-term rapid increases in 2-MIB concentrations in Eastern Taihu Lake, likely due to sediment resuspension. These findings provide a theoretical basis for managing 2-MIB in Eastern Taihu Lake.