The research on biological monitoring based on eDNA technology has witnessed rapid development and expansion in recent years, presenting a technological revolution trend that is partially replacing traditional field survey methods. In the vigorous expansion where so many researchers are competing to use eDNA technology for research and publishing papers, there are a series of risks that may undermine the foundation of these conclusions and the value of these papers. For instance, what object exactly does the eDNA monitoring result indicate? Is it appropriate to describe and analyze the eDNA monitoring results using traditional ecological concepts and methods applicable to individual survey data analysis? To explore this issue, the current manuscript took the applicability of the extended application of quantitative indicators from traditional community ecology in analyzing eDNA monitoring results as the starting point for its research. Four questions were analyzed. Firstly, what exactly does the relative abundance of DNA sequences of each detected species reflect in the eDNA monitoring results? Secondly, what exactly do the community quantitative indicators calculated based on the relative abundance of DNA sequences of each detected species indicate in the eDNA monitoring results? Thirdly, is it effective that using the relative abundance of DNA sequences of each detected species to replace the proportion of individual numbers or biomass in calculating community quantitative indicators? Fourthly, how to construct the concept system and terminology system for indicating and describing the community quantitative indicators calculated based on the detected species and the relative abundance of DNA sequences of each detected species reflect in the eDNA monitoring results? Then, it proposed three suggestions for the result description and interpretation in the biological monitoring and community analysis based on eDNA technology. The first, carefully describe the results of eDNA monitoring or investigation, and do not indiscreetly present them using the same methods for the results of traditional investigation and monitoring. The second, scientifically understand what the eDNA monitoring or investigation results indicate, and avoid ambiguity and confusion in the interpretation and discussion of the results. The third, get a growing consensus on this issue and construct the concept system and terminology system for indicating and describing the results of all research fields based on eDNA technology according to gradually explore and study. The current manuscript merely presents a preliminary exploration of the fundamental questions in the eDNA monitoring and analyzing, hoping to serve as a stimulus for further discussion.