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| 引用本文: | 郝怀庆,施国新,杜开和,徐勤松.Hg2+对水鳖(Hydrocharis dubia)叶片生理生化及超微结构的毒害效应.湖泊科学,2001,13(2):163-168. DOI:10.18307/20010210 |
| HAO Huaiqing,SHI Guoxin,DU Kaihe,XU Qinsong.Toxic Effect of Hg2+ on Physiology and Ultrastructure of Hydrocharis dubia. J. Lake Sci.2001,13(2):163-168. DOI:10.18307/20010210 |
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| Hg2+对水鳖(Hydrocharis dubia)叶片生理生化及超微结构的毒害效应 |
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郝怀庆, 施国新, 杜开和, 徐勤松
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南京师范大学生命科学学院, 南京 210097
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| 摘要: |
| 研究了不同浓度Hg2+对水鳖叶片光合放氧速率、呼吸速率、细胞膜透性、硝酸还原酶活性以及超微结构的毒害影响.结果表明,随着Hg2+浓度的增加,水鳖叶片的光合放氧速率逐渐下降,呼吸速率、硝酸还原酶活性先升后降,而细胞膜透性则逐渐上升.电镜观察发现,水鳖叶细胞遭受Hg2+毒害初期,染色质凝集,叶绿体中类囊体片层以及线粒体嵴突膨胀.随着叶细胞遭受毒害程度的加深,核膜破裂,染色质与核质消失,叶绿体膨胀解体,线粒体空泡化 |
| 关键词: 水鳖 Hg2+ 光合放氧速率 细胞膜透性 硝酸还原酶 超微结构 |
| DOI：10.18307/20010210 |
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| 基金项目:国家自然科学基金(39770046);江苏省自然科学基金(BK7107)共同资助 |
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| Toxic Effect of Hg2+ on Physiology and Ultrastructure of Hydrocharis dubia |
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HAO Huaiqing, SHI Guoxin, DU Kaihe, XU Qinsong
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College of Life Science Nanjing Normal University Nanjing 210097, P. R. China
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| Abstract: |
| The toxic effect of various concentrations of Hg2+ on the photosythentic O2 evolution respiration rate the permeability of cell membrane nitrate reductase activity and the ultrastructure changes in leaf cells of Hydrocharis dubia were studied. The results showed that the photosythentic O2 evolution decreased respiration rate and nitrate reductase activity first increased and then decreased while the permeability of cell membrane was increased. Lltrastructure observation revealed that the chromatin condensed the thylakoids of chloroplast and cristae of mitochondria swelled at the early stage when the leaves suffered the poison of Hg2+. With the increasing of the poison nuclear membrane disrupted the chromatin and nucleoplasm disappeared, chloroplast disintegrated and mitochondria vesiculated. |
| Key words: Hydrocharis dubia (BL.)Backer Hg2+ photosythentic O2 evolution permeability of cell membrane nitrate reductase ultrast ructure |
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