|
| 引用本文: | 黄代中,肖文娟,刘云兵,刘竟远,周易勇.浅水湖泊沉积物脱氢酶活性的测定及其生态学意义.湖泊科学,2009,21(3):345-350. DOI:10.18307/2009.0305 |
| HUANG Daizhong,XIAO Wenjuan,LIU Yunbing,LIU Jingyuan,ZHOU Yiyong.Determination of dehydrogenase activity in sediment of shallow lakes and its ecological significance. J. Lake Sci.2009,21(3):345-350. DOI:10.18307/2009.0305 |
|
| |
|
|
| 本文已被：浏览 8042次 下载 4916次 |
码上扫一扫！ |
|
|
| 浅水湖泊沉积物脱氢酶活性的测定及其生态学意义 |
|
黄代中1,2, 肖文娟1,2, 刘云兵1,2, 刘竟远1, 周易勇1
|
|
1.中国科学院水生生物研究所, 武汉 430072;2.中国科学院研究生院, 北京 100049
|
|
| 摘要: |
| 以武汉市浅水湖泊(月湖)为实验对象确定了沉积物脱氢酶活性(DHA)测定的最佳条件:以经甲醛灭菌的沉积物为对照,以氯化三苯基四氮唑为电子受体(0.4%),沉积物用量为0.5g,pH值为7.5,培养时间为3h.据上述条件测定的DHA比未优化条件下的相应值高3-5倍,且表现出与沉积物有机质含量更为显著的正相关关系.武汉东湖3个子湖共23个采样点沉积物样品的测试结果进一步证实了上述方法的可行性,同时初步揭示了湖泊沉积物脱氢酶反映微生物活性与有机质状态的生态学意义. |
| 关键词: 沉积物 脱氢酶 TTC 微生物活性 有机质 湖泊 |
| DOI：10.18307/2009.0305 |
| 分类号: |
| 基金项目:国家“973”计划(2008CB418005);中国科学院知识创新工程重要方向项目(KZCX2-YW-426-01);中国科学院知识创新工程重大交叉项目(KZCX1-YW-14-1) |
|
| Determination of dehydrogenase activity in sediment of shallow lakes and its ecological significance |
|
HUANG Daizhong1,2, XIAO Wenjuan1,2, LIU Yunbing1,2, LIU Jingyuan1, ZHOU Yiyong1
|
|
1.Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, P. R. China;2.Graduate School of Chinese Academy of Sciences, Beijing 100049, P. R. China
|
| Abstract: |
| The samples were taken from the shallow urban lakes(Lake Yuehu) in Wuhan city to optimize the determination of dehydrogenase activity (DHA) in sediment, which gave the following suitable conditions: the sediment sterilized by formaldehyde was used as the control, the concentration of electron acceptor (2,3,5-triphenyl-2H-tetrazolium chloride) was 0.4%, the sediment amount used was 0.5g, pH value was 7.5 and the reaction time was 3h. Under these conditions, the DHA assayed was 3-5 times higher, and its positive relation to the content of organic matter in sediment was more significant, compared to those determined by the unmodified method. The data based on the 23 sites from 3 basins of Lake Donghu demonstrated the feasibility of the method. In the same time, the ecological significance of DHA was further shown in terms of the measurements of microbial activities and organic matter status in lake sediment |
| Key words: Sediment dehydrogenase TTC microbial activities organic matter lake |
|
|
| 参考文献(共29条): | | [1] Huan Xin Weng, Ya Chao Qin, Xiang Wei Sun et al. Iron and phosphorus effects on the growth of Cryptomonas sp.(Cryptophyceae) and their availability in sediments from the Pearl River Estuary, China. Estuarine, Coastal and Shelf Science, 2007, 73(3-4): 501-509. | | [2] Sinclair DCR, Smith GM, A Bruce HJ Staines. Soil dehydrogenase activity adjacent to remedially treated timber, weathered in a physical field model. International Biodeterioration & Biodegradation, 1997, 39(2-3): 207-216. | | [3] Beata Keplin, Gabriele Broll. Earthworms and dehydrogenase activity of urban biotopes. Soil Biol Biochem, 1997, 29(3-4): 533-536. | | [4] Sonia M Tiquia, Judy HC Wan, Nora FY Tam. Dynamics of yard trimmings composting as determined by dehydrogenase activity, ATP content, arginine ammonification, and nitrification potential. Process Biochemistry, 2002, 37: 1057-1065. | | [5] Mei Sheng Xia, Cai Hong Hu, Hong Mei Zhang. Effects of tourmaline addition on the dehydrogenase activity of Rhodopseudomonas palustris. Process Biochemistry, 2006, 41: 221-225. | | [6] Sophie Chaperon, Sebastien Sauve. Toxicity interaction of metals (Ag, Cu, Hg, Zn) to urease and dehydrogenase activities in soils. Soil Biology & Biochemistry, 2007, 39: 2329-2338. | | [7] Kızılkaya R. Dehydrogenase activity in Lumbricus terrestris casts and surrounding soil affected by addition of different organic wastes and Zn. Bioresour Technol, 2008, 99(5): 946-953. | | [8] Sushma Pandey, Dileep K Singh. Soil dehydrogenase, phosphomonoesterase andarginine deaminase activities in an insecticide treated groundnut (Arachis hypogaea L) field. Chemosphere, 2006, 63: 869-880. | | [9] Jitendra Singh, Dileep K Singh. Dehydrogenase and phosphomonoesterase activities in groundnut (Arachis hypogaea L) fieldafter diazinon, imidacloprid and lindane treatments. Chemosphere, 2005, 60: 32-42. | | [10] Lenhard G, Ross WR, A DU Plooy. A study of methods for the classification of bottom deposits of natural waters. Hydrobiologia, 1962, 20: 223-240. | | [11] Mario M Pamatmat, Ashok M Bhagwat. Anaerobic metabolism in lake Washington sediment. Limnology and Oceanography, 1973, 18(4): 611-627. | | [12] Neto M, Ohannessian A, Delolme C et al. Towards an optimized protocol for measuring global dehydrogenase activity in storm-water sediments. J Soils Sediments, 2007, 7(2): 101-110. | | [13] Kimio Hirabayashi, Kazuya Yoshizawa, Norihiko Yoshida et al. Progress of eutrophication and change of chironomid fauna in Lake Yamanakako, Japan. Limnology, 2004, 5: 47-53. | | [14] Gary E Jenneman, Michael J Mcinerney, Michael E Crocker et al. Effect of sterilization by dry heat or autoclaving on bacterial penetration through berea sandstone. Applied and Environmental Microbiology, 1986, 51(1): 39-43. | | [15] Trevors JT. Sterilization and inhibition of microbial activity in soil. Journal of Microbiological Methods, 1996, 26: 53-59. | | [16] Von Mersi, Schinner WF. An improved and accurate method for determining the dehydrogenase activity of soils with iodonitrotetrazolium chloride. Biol Fertil Soils, 1991, 11: 216-220. | | [17] Camina F, Trasar cepeda C, Gil sotres F et al. Measurement of dehydrogenase activity in acid soils rich in organic matter. Soil Biology and Biochemistry, 1998, 30(8-9): 1005-1011. | | [18] Prenger JP, Reddy KR. Microbial enzyme activities in a freshwater marsh after cessation of nutrient loading. Soil Science Society of America Journal, 2004, 68(5): 1796-1804. | | [19] Rolda\'n A, Salinas Garcı\'a JR, Alguacil MM et al. Soil enzyme activities suggest advantages of conservation tillage practices in sorghum cultivation under subtropical conditions. Geoderma, 2005, 129: 178-185. | | [20] 朱南文, 闵 航, 陈美慈等. TTC-脱氢酶测定方法的探讨. 中国沼气, 1996, 14(2): 3-5. | | 更多... |
|
|
|
|
|
|
|
