| 摘要: |
| 随着基因测序技术的发展，环境中一些微藻不断被发现，超微蓝藻便是其中之一，但关于超微蓝藻的多相分类学研究在我国报道较少。本研究首次在某自来水厂分离纯化获得了一株超微蓝藻藻株Cyanobium gracile HNU001，并对其形态学、超微结构和分子特征进行了研究。形态学发现其藻细胞呈椭圆形或椭球形，在分裂过程中平行于短的细胞轴对称存在；超微结构显示了细胞二分裂繁殖以及类囊体膜平行于细胞膜排列；16S rRNA基因序列显示该藻株和双色藻属Cyanobium的序列相似性大于99.9%，分子系统树聚为一枝；该藻株和Cyanobium gracile PCC 6307的D1-D1’、Box-B螺旋结构一致。综合以上结果，将其命名为纤细双色藻（Cyanobium gracile），是在我国的首次分类学描述。 |
| 关键词: 蓝藻 超微蓝藻 双色藻 分类 分子系统 |
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| Cyanobium(Synechococcales, Cyanobacteria), a new recorded genus of terrestrial cyanobacteria in China |
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liu yang, wei ya fei
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| Abstract: |
| With the advancement of gene sequencing technology, researchers have been able to uncover previously unknown microalgae in the environment, such as picocyanobacteria. Despite this, there is a scarcity of literature on the comprehensive taxonomic analysis of picocyanobacteria in China. This study focused on the isolation and purification of Cyanobium gracile HNU001 from a waterworks, marking the first time this species has been studied in such detail. The research delved into the morphology, ultrastructure, and molecular characteristics of the organism. Morphological observations revealed ellipsoidal or elongated algal cells that divided symmetrically into shorter cells. Ultrastructural analysis indicated cell division through dimitosis and the arrangement of thylakoid membranes parallel to the cell membrane. Analysis of the 16S rRNA gene sequences demonstrated a high similarity of over 99.9% between this strain and Cyanobium, placing it within a distinct molecular cluster. Comparative analysis of the D1-D1" and Box-B helical structures of Cyanobium gracile HNU001 with Cyanobium gracile PCC 6307 showed consistency. Based on these findings, the species was formally named Cyanobium gracile, marking the first taxonomic characterization in China. |
| Key words: cyanobacteria picocyanobacteria Cyanobium taxonomy phylogeny |
