| 摘要: |
| 用小球藻(Chlorella sp.)为食培养萼花臂尾轮虫(Brachionus calyciflorus),食物浓度约1×106cells/mL,温度25℃,光照强度约40001x,昼长比LD=18:6。在1.0mL、0.5mL和0.25mL培养液中同时进行单个体和群体培养。结果发现:1)单个体培养时每个母体平均产卵量分别为12.0,13.8和7.8个;后代个体中混交雌体的百分比分别是46.38%,53.49%和55.83%。2)群体培养时,每个母体平均产卵量分别为8.7、3.1和2.65个,随密度增加而减少;后代个体中混交雌体百分比分别为41.70%,53.59%和54.26%,群体培养的密度为4.0ind./mL与2.0ind./mL,2.0ind./mL与1.0ind./mL混交雌体的百分比差异不明显。母体密度高低与后代混交雌体百分比间具有明显相关性。在上述培养条件下,轮虫为1.0ind./mL时,进行单个体培养,分别用急剧冷休克(SS)、逐渐冷休克(GS)和恒温对照(C)进行处理,结果表明:个体平均产卵量分别为10.8、9.2和12.6个。后代混交雌体百分比分别为32.85、20.7和16.87,SS和C处间后其混交雌体比例具显著差异,而SS和GS,GS和C处理间没有显著差异。 |
| 关键词: 萼花臂尾轮虫 混交雌体 密度 温度 产卵量 |
| DOI：10.18307/1996.0412 |
| 分类号: |
| 基金项目:国家自然科学基金资助项目(编号:39270098) |
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| Effects of density and temperature on the egg and mictic female produced by brachionus calyciflorus (rotatoria: monogononta) |
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Yang Jiaxin, Huang Xiangfei
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Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072
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| Abstract: |
| Brachionus calyciflorus Pallas were cultured in both single and group rotifers in the medium of 1.0, 0.5 and 0.25 mL under the experimental conditions of 25℃, light intensity about 4000 Ix(LD=18:6) and the food concentration about 1.0×106 cells/mL of Chlorella sp.In single culture, the mean egg numbers per mother female were 12.0 ind., 13.8 ind.and 7.8 ind.The percentages of mictic female of offsprings were 46.38, 53.49, and 55.83 respectively.In group culture the mean egg numbers were 8.7,3.1 and 2.65 inds.,respectively.And the percentages of mictic females of offsprings were 41.70, 53.59 and 54.26 respectively.There were significant differences between the percentage of mictic females of offsprings and the densities of mother females (F=3.45,α0.05). Put one rotifer in every dish. one of the dishes was precooled to 5℃ as sudden cold shock (SS) treatment, and was kept for 2 hrs at 5±TC, another dish was cooled gradually from 25℃ to 5±1℃ during one hour,then was kept at 5±1℃ for one hour again,as the gradual cold shock (GS).After the treatment, both dishes were moved to 25℃, and cultured at 25±1℃ over the experiments, the third dish was left at 25℃ as contrast (C).The mean egg numbers produced by mother females were 10.8, 9.2 and 12.6 ind, respectively.The per-centages of mictic female of offsprings were 32.9, 20.7 and 16.87, respectively.The results show that the difference between SS and C is significant (t=2.16,α=0.05), but both differences between GS and SS, GS and C were not significant (t=l.32 and 0.52, α=0.05). |
| Key words: Brachionus ealyciflorus density temperature mictic female mean egg |
