| 引用本文: | 周易勇,李建秋,付永清,陈旭东,张玉敏.浅水湖泊中碱性磷酸酶活性及其动力学参数的分层现象.湖泊科学,2000,12(1):53-58. DOI:10.18307/2000.0108 |
| ZHOU Yiyong,LI Jianqiu,FU Yongqing,CHEN Xudong,ZHANG Yumin.Stratification of Activities and Kinetics of Alkaline Phosphatase in A Shallow Chinese Freshwater Lake (Donghu Lake, Wuhan). J. Lake Sci.2000,12(1):53-58. DOI:10.18307/2000.0108 |
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| 摘要: |
| 1995年5月东湖与严西湖各采样点间隙水、上覆水和表层水中碱性磷酸酶的动力学参数(Vmax和Km值)明显不同(P<0.05).与表层水相比,东湖I站上覆水中酶的Vmax明显较高,严西湖各站上覆水中酶的Vmax明显较低,而在东湖Ⅱ站,上覆水中酶的Vmax和Km值明显较低,故仍有较高的催化效率.这-现象似与酶活性的组成有关、总之,富营养的东湖各站诸层次磷的酶促转换效率自上而下呈明显的递增趋势,严西湖则无此特征因此,富营养湖泊沉积物中磷的"内负荷"应有可酶解有机磷的含义,浅水湖泊中磷酸酶的活性组成和动力学参数的分层现象当为湖沼学研究中值得深入探讨的新理论问题. |
| 关键词: 分层 碱性磷酸酶 动力学 浅水湖泊 |
| DOI：10.18307/2000.0108 |
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| 基金项目:国家自然科学基金(39670149、39170165);淡水生态与生物技术国家重点实验室基金;中国科学院东湖湖泊生态系统试验站、中国科学院长春地理研究所现代湿地过程与环境开放实验室基金以及中国科学院"青年科学家小组"基金联合资助项目 |
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| Stratification of Activities and Kinetics of Alkaline Phosphatase in A Shallow Chinese Freshwater Lake (Donghu Lake, Wuhan) |
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ZHOU Yiyong, LI Jianqiu, FU Yongqing, CHEN Xudong, ZHANG Yumin
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Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, P. R. China
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| Abstract: |
| In May 1995, the kinetic parameters (Vmax and Km) of alkaline phosphatase in interstitial water, overlying water and surface water were differed significantly in Donghu Lake (Wuhan) and Yanxihu Lake(P<0.05).At Station I and HI of Donghu Lake, Vmax values of overlying water were much higher than those of surface water, while in Yanxihu Lake Vmax values of overlying water were much lower.At Station II in Donghu Lake, both Vmax and Km values were quite lower in overlying water, exhibiting a higher catalytic efficiency.This phenomenon might relate with the compositions of enzyme activities.In brief, a pronounced downward gradient of enzyme catalytic efficiency was observed in Donghu Lake, which was not recorded in Yanxihu Lake.Consequently, the enzymatic hydrolyzable P may contribute to the internal loading of the eutrophic lake, and stratification of kinetics and compositions of phosphatase in shallow lake is theoretically noticeable. |
| Key words: Stratification alkaline phsophatase kinetics shallow lake |
