| 引用本文: | 杨顶田,施国新,宋东杰,陈伟民.莼菜(Brasenia schreberi)冬芽对Cr6+污染的抗性反应.湖泊科学,2001,13(2):169-174. DOI:10.18307/20010211 |
| YANG Dingtian,SHI Guoxin,SONG Dongjie,CHEN Weimin.The Resistant Reaction of Brasenia schreberi Winter-Bud to Cr6+ Pollution. J. Lake Sci.2001,13(2):169-174. DOI:10.18307/20010211 |
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| 摘要: |
| 研究了Cr6+污染对莼菜冬芽外部形态及过氧化物酶、硝酸还原酶活性、叶绿素、O2-·、MDA的含量等生理指标的影响.结果显示:Cr6+对莼菜毒害机制在于抑制酶活性和损害细胞膜系统.在培养第三天时叶绿素的含量随着Cr6+浓度的增加而降低;过氧化物酶的活性在10mg/L中最高;硝酸还原酶活性除在5mg/L中的略微升高外,在10- 40mg/L之间随着Cr6+培养浓度的升高而降低;O2-·的含量在5mg/L中的最高,在10mg/L中略高于对照,在随后培养中随浓度的升高而降低,MDA的含量变化趋势和O2-·相似 |
| 关键词: 莼菜 Cr6+ 过氧化物酶 硝酸还原酶 O2-· |
| DOI：10.18307/20010211 |
| 分类号: |
| 基金项目:国家自然科学基金资助项目(39770046);江苏省自然科学基金资助项目(BK97107) |
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| The Resistant Reaction of Brasenia schreberi Winter-Bud to Cr6+ Pollution |
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YANG Dingtian1, SHI Guoxin2, SONG Dongjie2, CHEN Weimin1
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1.Nanjing Institute of Geography and Limnology, Chinese Academy of Sciences, Nanjing 210008, P. R. China;2.Nanjing Normal University Nanjing 210097, P. R. China
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| Abstract: |
| This paper mainly deals with the changes of external morphology, the activity of peroxidase and nit rate reductase, the content of chlorophyll, O2-· and MDA in Brasenia schreberi by Cr6+ treatment.The results show ed the reason of Cr6+ injuring Brasenia schreberi w as that Cr6+ inhibited the activity of enzymes and damaged membrane.In the third day, the content of chlorophyll reduced with the increasing concent ration of Cr6+, the activity of peroxidase was the highest at 10 mg/L, the activity of nit rate reductase was a lit tle higher at 5mg/L, but reduced with the increasing concent ration of Cr6+ up to 40mg/L, the content of O2-· w as the highest at 5mg/L, and a little higher than control group at 10mg/L, then it reduced with the increasing culture concentration of Cr6+, the trend of changes in MDA was relatively similar with that of O2-·. |
| Key words: Brasenia schreberi Cr6+ peroxidase nitrate reductase O2-· |
