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引用本文:胡章立,邢苗,吴玉荷,涂欢.转MT-like基因衣藻的重金属结合能力与抗性特征分析.湖泊科学,2002,14(3):247-252. DOI:10.18307/2002.0309
HU Zhangli,XING Miao,WU Yuhe,TU Huan.The Comparison of Heavy Metal Binding Properties and Tolerance between Transgenic Strains and Wild Type in Chlamydomonas reinhardtii. J. Lake Sci.2002,14(3):247-252. DOI:10.18307/2002.0309
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转MT-like基因衣藻的重金属结合能力与抗性特征分析
胡章立, 邢苗, 吴玉荷, 涂欢
深圳大学生命科学学院, 深圳518060
摘要:
通过对转MT-like基因衣藻和其野生品系的比较分析,发现转基因藻株对重金属镉和铜的结合能力及抗性均有大幅提高.在重金属结合能力方面,低浓度 (5~10 μmol/L)的镉溶液中,转基因藻重金属结合量是野生株的 1.5倍左右,而相对高浓度 (30~5 0 μmol/L)的镉溶液中,由于重金属对藻细胞的毒害作用,使两者的差异不明显.在重金属铜和镉的抗性方面,转基因藻表现出明显高于野生藻株的抗性特征.用转基因藻去处理含铜的工业废水,结果发现转基因藻的处理效率可以提高 10%以上.这说明DNA重组技术对提高藻类的废水处理效率方面具有较大的潜力.
关键词:  DNA重组  莱茵衣藻  废水处理
DOI:10.18307/2002.0309
分类号:
基金项目:广东省自然科学基金(011743);深圳市科技局基金联合资助
The Comparison of Heavy Metal Binding Properties and Tolerance between Transgenic Strains and Wild Type in Chlamydomonas reinhardtii
HU Zhangli, XING Miao, WU Yuhe, TU Huan
College of Life Science, Shenzhen University, Shenzhen 518060, P.R.China
Abstract:
The Comparison of the heavy metal binding properties and tolerance was studied between wild type and transgenic strains which expressed a foreign MT-like gene from Festuca rubraL Merlin in Chlamy domonas reinhardtii.The results showed that the cadmium binding capacity of transgenic strain was 1.5-fold of the wild type in low cadmium concentration(5-10μmol/L), however, there was not marked difference of heavy metal binding capacity in high cadmium concentrtion (30-50μmol/L).Itindicated that the difference of heavy metal binding capacity was from active uptake between transgenic strain and wild type.On the other hand, transgenic strain also showed more higher tolerance than wild type when they were cultured in 150μmol/L copper solution medium and 600μmol/L cadmium agarmedium.Transgenic strain was used to treat wastewater, it could enhance more than 10% of copper removal efficiency than wild type.
Key words:  DNA recombination  Chlamydomonas reinhardtii  wastewater treatment
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