| 引用本文: | 王山杉,刘永定,金传荫,李敦海.Zn2+浓度对固氮鱼腥藻(Anabaena azotica Ley)光能转化特性的影响.湖泊科学,2002,14(4):350-356. DOI:10.18307/2002.0409 |
| WANG Shanshan,LIU Yongding,JIN Chuanyin,LI Dunhai.Effects of Zinc Ion on Photosynthetic System of Anabaena azotica Ley. J. Lake Sci.2002,14(4):350-356. DOI:10.18307/2002.0409 |
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| 摘要: |
| 本实验对在不同Zn2+浓度条件下培养的固氮鱼腥藻(Anabaena azotica Ley)的生长、光合放氧速率和叶绿素荧光参数Fv/Fm进行了测定.结果表明,当Zn2+浓度为1.0μmol/L时,其比生长速率(Specific growth rate)最大,光合放氧速率和Fv/Fm值最高.当Zn2+浓度大于等于5.0μmol/L时会抑制A.azotica Ley的生长和光合作用.对在0μmol/L和5.0μmol/L Zn2+浓度下生长的藻细胞藻胆体-类囊体膜复合物吸收光谱的比较和对与5.0μmol/L Zn2+发生反应的藻蓝蛋白溶液的可见光吸收光谱的分析,发现前者624nm处藻胆体的吸收峰和后者620nm处藻蓝蛋白的吸收峰都因Zn2+的作用而下降,推测藻蓝蛋白为Zn2+影响光合作用的位点之一.碳酸酐酶活性的测定表明Zn2+的浓度水平会影响其活性大小,推测是Zn2+影响光合作用的另-途径. |
| 关键词: 固氮鱼腥藻 锌离子 光合作用 藻蓝蛋白 碳酸酐酶 |
| DOI：10.18307/2002.0409 |
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| 基金项目:国家湖泊重点研究课题(K99-05-35-01)资助;中国科学院水生生物研究所创新领域前沿项目(220316)资助 |
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| Effects of Zinc Ion on Photosynthetic System of Anabaena azotica Ley |
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WANG Shanshan, LIU Yongding, JIN Chuanyin, LI Dunhai
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Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, P. R. China
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| Abstract: |
| It was found that the concentration of Zn2+< at about 1.0μm0l/L was essential to keep optimal growth of Anabaena azotica Ley and also important to keep a quite high value of photosynthetic oxygen evolution rate, chlorophyll fluorescence (Fv/Fm) level and carbonic anhydrase activity, whereas the relative high concentration of Zn2+(≥5.Oμm) would inhibit the growth of A.azotica Ley, decrease its photosynthetic oxygen evolution rate, chlorophyll fluorescence and CA activity.The study of the absorption spectra of the phycobilisome-thy-lakoid membrane complex and the extraction of phycocyanin indicate that Zn2+ may affect the photosynthesis by its effect on the phycobilisome especially the phycocyanin.Since CA is a Zn2+-containing metalloenzyme and may provide a high concentration of CO2 to Rubisco in the process of CO2 fixation, it is suggested that Zn2+ may affect the photosynthesis by acting on CA. |
| Key words: Anabaejia azotica Ley Photosynthesis Zinc Ion Phycocyanin Carbonic Anhydrase |
