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引用本文:孙继贤,廖家遗.罗氏沼虾胸神经节中促进卵母细胞发育的激素的初步分离.湖泊科学,2003,15(1):64-69. DOI:10.18307/2003.0109
SUN Jixian,LIAO Jiayi.Preliminary Isolation of A Hormone Stimulating Oocyte Development from Thoracic Ganglion of the Prawn, Macrobrachium rosenbergii. J. Lake Sci.2003,15(1):64-69. DOI:10.18307/2003.0109
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罗氏沼虾胸神经节中促进卵母细胞发育的激素的初步分离
孙继贤, 廖家遗
中山大学生命科学学院, 广州 510275
摘要:
罗氏沼虾成虾的胸神经节匀浆经Sephadex-G50柱层析所得第2个峰再经HPLC分离得到9个组分.第1个组分和罗氏沼虾期卵巢小块一起体外培养36h,可使卵母细胞的直径较对照组非常显著地增大(P<0.01),这种增大卵母细胞直径的能力随该组分中蛋白质浓度的增加而增加.在该组分中的激素分子量在1.3kDa左右.结果显示罗氏沼虾胸神经节能分泌促进卵母细胞发育的激素.
关键词:  罗氏沼虾  胸神经节  卵母细胞发育  激素分离
DOI:10.18307/2003.0109
分类号:
基金项目:国家自然科学基金资助项目30070105.
Preliminary Isolation of A Hormone Stimulating Oocyte Development from Thoracic Ganglion of the Prawn, Macrobrachium rosenbergii
SUN Jixian, LIAO Jiayi
School of Life Sciences, Zhongshan University, Guangzhou 510275, P. R. China
Abstract:
The extracts of thoracic ganglion from adult Macrobrachium rosenbergii were chromatographed on a Sephadex G-50 column, the resulting second peak was further isolated by HPLC, and 9 fractions were obtained. Among the fractions only the first fraction significantly increased the oocyte diameter in vitro of the stage Ⅲ ovary in M. rosenbergii. The increasing effects were depended on the protein concentration of the fraction for culture. The molecular weight of the substance in the fraction 1 was about 1.3kDa.The results show that the thoracic ganglion of M. rosenbergii can secrete peptide hormone which stimulates the oocyte development of the prawn.
Key words:  Macrobrachium rosenbergii  thoracic ganglion  oocyte development  Hormone  isolation
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