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引用本文:王进,李建宏,华秀红,周逸芳.吞噬微囊藻的鞭毛虫的培养.湖泊科学,2005,17(2):183-187. DOI:10.18307/2005.0215
WANG Jin,LI Jianhong,HUA Xiuhong,ZHOU Yifang.Culture of a Microcystis-eating flagellate. J. Lake Sci.2005,17(2):183-187. DOI:10.18307/2005.0215
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吞噬微囊藻的鞭毛虫的培养
王进, 李建宏, 华秀红, 周逸芳
南京师范大学生命科学学院, 南京210097
摘要:
从一个有水华发生的池塘里采集分离到一种能吞噬微囊藻的动基体目鞭毛虫(Kinetoplastida),存光学显微镜及激光共聚焦显微镜下观察了该鞭毛虫的形态特征,初步探讨了它的培养方法.该鞭毛虫以集胞藻、微囊藻、鱼腥藻、小球藻以及蛋黄、奶粉、大肠杆菌和酵母为食物进行培养,结果集胞藻、微囊藻、奶粉、蛋黄、及酵母都是该鞭毛虫很好的食物.该鞭毛虫易于培养,繁殖迅速,培养成本低廉,有应用于生态环境中控制微囊藻水华的可能.
关键词:  异养鞭毛虫  微囊藻  胞吞作用  浮游动物
DOI:10.18307/2005.0215
分类号:
基金项目:教育部归国留学人员基金(2003)江苏省教委基金(03KJD180113)联合资助.
Culture of a Microcystis-eating flagellate
WANG Jin, LI Jianhong, HUA Xiuhong, ZHOU Yifang
College of Life Sciences, Nanjing Normal University, Nanjing 210097 P. R. China
Abstract:
A heterotrophic nanoilagellate (Kinetoplastida) was separated which feeds on Microcystis cells by endocytosis.Its morphological characters were observedd by the optical microscope and confocal scanning light microscope.The eating process could be finished in several seconds (even less than 3 seconds).One flagellate could eal more than ten Microcystis cells at most.The flagellate was single cell protozoo with one, two or four flagella.The flagellate was transparent and without pigments.It appeared to be spherical in shape at most of the time and occasionally they are elliptical or other shape.The spherical flagelltes ate Microcystis cells with low moving velocity, the other flagellates moved faster but diclnt eal Microcystis cells.The flagellate reproduced by equal binary fission and this procedure lasted about 5 minutes.According to the niorf)hological obsmation, the flagellate should be belonged to Sarcoraastigophorea, Zoomastigophorea, Kinetoplastida.The relative activity of protease was greatly unproved after inducing by feeding with Microcrstui cells.The results suggested that the flagellate have strong ability to digest the cells of Microcystis.It could remove 80% of tlie Microcystis cells in less than 20h in our study.The flagellate should be used for ecological control of Microcystis water bloom.To get a large population, the culturing of ihe flagellate was studied.SynechocystLs, Murocrstis, Anabaena, Chiorella, E.coh, yeast cells and yolk, milk powder were used as feed, respectively.Results showed that Syiiechocystis, Microcystis, milk power, yolk and yeast were its favorite foods.Among them, Syiiechocystis and yeast cells were better than others.The flagellate was endurable to low temperature.Even storing at 4℃ more than a month, it could survive aud reproduce as usual.The applicalion of this flagellate to natural ecosystem will be investigated rn futwre research.
Key words:  Flagellates  Microcystis  endocytosis  water bloooi
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