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引用本文:陈翠珍,房海,张晓君,葛慕湘,王秀云,靳晓敏.中华绒螯蟹病原鳗利斯顿氏菌生物学特性.湖泊科学,2006,18(3):293-298. DOI:10.18307/2006.0316
CHEN Cuizhen,FANG Hai,ZHANG Xiaojun,GE Muxiang,WANG Xiuyun,JIN Xiaomin.Biological characterization of Listonella anguillarum isolated from Crab (Eriocheir sinensis L.). J. Lake Sci.2006,18(3):293-298. DOI:10.18307/2006.0316
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中华绒螯蟹病原鳗利斯顿氏菌生物学特性
陈翠珍, 房海, 张晓君, 葛慕湘, 王秀云, 靳晓敏
河北科技师范学院动物科学系, 秦皇岛 066600
摘要:
从一起自然发病的中华绒螯蟹(Eriocheir sinensis H.Milne-Edwards)肝胰腺中,分离做纯培养的6株(HQ010516A-1至HQ010516A-6)鳗利斯顿氏菌(Listonella anguillarum),进行了形态特征、主要理化特性等方面的检验.同时择代表菌株(HQ010516A-1)测定了16SrRNA基因序列、构建了系统发育树.另外,以代表菌株(HQ010516A-1)对健康蟹进行了人工感染试验,结果表明了该菌株在所检病例的病原学意义;用37种抗菌类药物对3株菌所做的药敏试验,结果均表现对头孢噻肟等29种药物呈现敏感或高度敏感(抑菌圈直径在16-40mm)、对头孢唑啉等3种药物呈低度敏感(抑菌圈直径在10-12mm)、对青霉素G等5种药物耐药(无抑菌圈形成).
关键词:  中华绒螯蟹  鳗利斯顿氏菌  生物学特性
DOI:10.18307/2006.0316
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Biological characterization of Listonella anguillarum isolated from Crab (Eriocheir sinensis L.)
CHEN Cuizhen, FANG Hai, ZHANG Xiaojun, GE Muxiang, WANG Xiuyun, JIN Xiaomin
Department of Animal Science, Hebei Normal University of Science and Technology, Qinhuangdao 066600, P. R. China
Abstract:
Examination of L.anguillarum (6 strains:HQ010516A-1 to HQ010516A-6) which were isolated from liver of naturally infected crab (Eriocheir sinensis L. ) was conducted including the morphological characteristics, main physiological and biochemical characteristics, 16S rRNA gene sequence was detected and phylogenetic analysis was constructed. In the same time,artificial infection experiment of representative strain (HQ010516A-1) to healthy crab was conducted, the results showed corresponding pathogenicity of the strain. The tests of antibiotic sensitivity of 3 strains to used 37 antimicrobial agents showed that all strains were sensitive or high sensitive to 29 agents including cefotaxime et al. ( Diameter of the zones of inhibition was 16-40 mm),were slight sensitive to 3 agents including cefazolin et al. ( Diameter of the zones of inhibition was 10-12 mm),were resistant to 5 agents including penicillin G et al. ( There was no zones of inhibition).
Key words:  Crab ( Eriocheir sinensis L. )  Listonella anguillarum  Biological characterization
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