| 引用本文: | 马洪雨,岳永生,郭金峰,公维华,王慧.山东省三个鲤鱼群体遗传多样性及亲缘关系的微卫星标记分析.湖泊科学,2006,18(6):655-660. DOI:10.18307/2006.0615 |
| MA Hongyu,YUE Yongsheng,GUO Jinfeng,GONG Weihua,WANG Hui.Microsatellite marker analysis of genetic diversity and phylogenetic relationships in three populations of Cyprinus carpio L. y Shandong Province. J. Lake Sci.2006,18(6):655-660. DOI:10.18307/2006.0615 |
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| 摘要: |
| 利用微卫星标记技术对3个鲤鱼群体(DP、WS和SY)的遗传多样性及亲缘关系进行研究.结果表明.3个群体的遗传多样性总体水平较高,其中SY群体最高,WS群体次之,DP群体最低.根据基因型频率(P)检验了各位点的Hardy- Weinberg平衡情况.Fis值表明3个群体中共有32个微卫星位点的杂合度观测值过剩.3个群体间,WS和SY群体间的遗传相似系数最大(0.8320),遗传距离最小(0.1680),表明这两个群体亲缘关系较近;WS和DP群体间的遗传相似系数最小(0.8288),遗传距离最大(0.1712),表明这两个群体亲缘关系较远. |
| 关键词: 鲤鱼 微卫星 遗传多样性 亲缘关系 |
| DOI：10.18307/2006.0615 |
| 分类号: |
| 基金项目:国家科技部资助项目(04033810);山东省科技厅资助项目(04038335)共同资助. |
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| Microsatellite marker analysis of genetic diversity and phylogenetic relationships in three populations of Cyprinus carpio L. y Shandong Province |
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MA Hongyu1, YUE Yongsheng1, GUO Jinfeng1, GONG Weihua2, WANG Hui1
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1.Molecular genetics and animal breeding Lab, Shandong Agricultural University, Tai'an 271018, P. R. China;2.Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100094, P. R. China
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| Abstract: |
| The genetic diversity and the phylogenetic relationships of three Cyprinus carpio L. populations were studied by using microsatellite marker technology. It showed that the genetic diversity level of three Cyprinus carpio L. populations was high, population was the highest, WS population was lower,and DP population was the lowest. In order to check the depature of microsatellite loci from HWE, the genotypic frequencies were calculated. Furthermore,32 cases of observed heterozygosity excess(Fis <0) were observed in three populations. The genetic similarity index between WS population and SY population was the highest(0.8320) among the three populations, and their genetic distance was the lowest (0.1680),which indicated that the phylogenetic relationship between these two populations was quite close. On the other hand, the genetic similarity index between WS population and DP population was the Iowest(0.8288),and their genetic distance was the highest(0.1712),which indicated that the phylogenetic relationship between ihese two populations was quite far. |
| Key words: Cyprinus carpio L. microsatellite genetic diversity phylogenetic relationship |
