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引用本文:张光富,高邦权.江浙莼菜遗传多样性和遗传结构的ISSR分析.湖泊科学,2008,20(5):662-668. DOI:10.18307/2008.0516
ZHANG Guang-fu,GAO Bang-quan.Analysis on genetic diversity and genetic structure of Brasenia schreberi in Jiangsu and Zhejiang Provinces revealed by ISSR Markers. J. Lake Sci.2008,20(5):662-668. DOI:10.18307/2008.0516
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江浙莼菜遗传多样性和遗传结构的ISSR分析
张光富1,2, 高邦权1
1.南京师范大学生命科学学院, 南京 210046;2.中国科学院南京地质古生物研究所, 现代古生物学和地层学国家重点实验室, 南京 210008
摘要:
利用ISSR标记对分布于江苏和浙江的濒危水生植物莼菜(Brasenia schreberi J.F.Gmelin)的遗传多样性和遗传结构进行研究.从77个引物中筛选出12个引物用于研究,共扩增出101条扩增带,其中多态性条带16条,占15.84%,平均每个引物扩增的条带数为8.4条.结果表明:莼菜的遗传多样性水平较低(种水平的遗传多样性Ht为0.0395,Shannon's信息指数Hs为0.0630;种群水平的遗传多样性Ht、Hs分别为0.0391、0.0335).大部分遗传变异存在于种群内(85.79%),种群间的基因流为3.0199.营养繁殖、生境丧失与片断化以及人工引种等人为干扰对莼菜的遗传多样性和遗传结构产生重要影响.
关键词:  莼菜  遗传多样性  遗传结构  ISSR
DOI:10.18307/2008.0516
分类号:
基金项目:江苏省教育厅项目(04KJD180105);现代古生物学和地层学国家重点实验室(中国科学院南京地质古生物研究所)(083111)联合资助
Analysis on genetic diversity and genetic structure of Brasenia schreberi in Jiangsu and Zhejiang Provinces revealed by ISSR Markers
ZHANG Guang-fu1,2, GAO Bang-quan1
1.School of Life Sciences, Nanjing Normal University, Nanjing 210046, P.R.China;2.State Key Laboratory of Palaeobiology and Stratigraphy, Nanjing Institute of Geology and Palaeontology, Chinese Academy of Sciences, Nanjing 210008, P.R.China
Abstract:
In order to investigate the level of genetic diversity of the endangered species Brasenia schreberi, three populations from Jiangsu and Zhejiang Provinces were sampled and analyzed using inter-simple sequence repeats (ISSR) markers. A total of fifty-four individuals were sampled. Twelve filtrated from 77 ISSR primers gave rise to 101 discernible DAN fragments of which 16 were polymorphic (15.84%). The average number of DNA bands amplified by each primer was 8.4. The Nei's genetic and Shannon's information indexes were 0.0395 and 0.0630 respectively at species level, 0.0391 and 0.0335 respectively at population level. Based on Nei's Gst value and Shannon's index, it showed that most of genetic diversity (85.79%) was within populations, and that gene flow (Nm) was 3.0199. It was concluded that vegetative propagation, habitat loss and fragment, introducing plants, and human disturbances may play a significant negative role on genetic diversity and genetic structure of Brasenia schreberi.
Key words:  Brasenia schreberi J. F. Gmelin  genetic diversity  genetic structure  ISSR
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