| 引用本文: | 宁华,张荣先,陈浩,杨志荣,孙群.滇池中芽孢杆菌的ARDRA分类及溶藻特性.湖泊科学,2008,20(5):675-680. DOI:10.18307/2008.0518 |
| NING Hua,ZHANG Rong-xian,CHEN Hao,YANG Zhi-rong& SUN Qun.ARDRA analysis of Bacillus spp. isolated from Lake Dianchi and their algae-lytic effect. J. Lake Sci.2008,20(5):675-680. DOI:10.18307/2008.0518 |
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| 摘要: |
| 针对从滇池中分离到的28株芽孢杆菌,用扩增rDNA限制性酶切片段分析法(ARDRA)进行了初步分类,共获得5个操作分类单元(OUT),其中OTU5为主要类型,包括14株细菌菌株.同时,以铜绿微囊藻和水华鱼腥藻为裂解对象,对该28株菌株的溶藻能力进行了测定,发现OTU5中有6株芽孢杆菌具有较好的溶藻能力,其培养液上清液具有强烈的溶藻作用,能在pH7、25℃的条件下抑制受试藻类的生长,可见,该6株芽孢杆菌应是通过分泌胞外物质而起溶藻作用的. |
| 关键词: 芽孢杆菌 ARDRA 溶藻细菌 溶藻作用 滇池 |
| DOI：10.18307/2008.0518 |
| 分类号: |
| 基金项目:四川省科技攻关计划高效生物杀藻剂的研究与应用项目(05NG002-006)资助 |
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| ARDRA analysis of Bacillus spp. isolated from Lake Dianchi and their algae-lytic effect |
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NING Hua1, ZHANG Rong-xian2, CHEN Hao3, YANG Zhi-rong& SUN Qun1
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1.College of Life Sciences, Sichuan University, Chengdu 610064, P.R.China;2.Tongren Polytechnic, Tongren 554300, P.R.China;3.Sichuan Institute of Atomic Energy, Chengdu 610066, P.R.China
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| Abstract: |
| A total of 28 Bacillius strains isolated from Lake Dianchi were classified by amplified rDNA restriction analysis (ARDRA) on their 16SrDNA through restriction digestion by AluI. Our data showed that all strains isolated could be classified into 5 Operational Taxonomic Units (OUT), and OTU5 was the dominant one, including 14 species. The lytic effects of all strains on Microcystis aeruginosa and Anabaena flos-aquae were studied, and 6 strains in OTU5 showed much stronger activity than others. The supernatant of 4 strains in OTU5 exhibited lytic function at almost the same strong level as their corresponding whole broth, which indicated that extracellular lytic substance produced by Bacillus cells could have lytic effects on algae. |
| Key words: Bacillus ARDRA algae-lytic bacteria algae-lytic function Lake Dianchi |
