| 引用本文: | 王捷,谢树莲,王中杰,徐瑶,李仁辉.汾河(太原市景区段)微囊藻的分子多样性及产毒能力.湖泊科学,2011,23(4):505-512. DOI:10.18307/2011.0403 |
| WANG Jie,XIE Shulian,WANG Zhongjie,XU Yao,LI Renhui.Molecular diversity and microcystin production of Microcystis in Fenhe River of Taiyuan. J. Lake Sci.2011,23(4):505-512. DOI:10.18307/2011.0403 |
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| 摘要: |
| 对太原市汾河景区采集到的微囊藻进行分离纯化, 得到7株微囊藻藻株, 运用ITS、PC-IGS和gyrB基因序列构建系统树来研究其分子多样性.结果表明, 太原市汾河景区微囊藻具有一定的分子多样性.采用mcyA基因和ELISA检测两种方法, 即全细胞PCR测定微囊藻毒素合成酶基因A(mcyA), 对这些藻株的微囊藻毒素进行测定, 检测的7株微囊藻中有6株含有mcyA基因, ELISA检测微囊藻细胞干粉产毒量在0.003-0.043mg/g之间, 属于产低毒藻种.本文是首次报道太原市汾河景区微囊藻产毒素, 也为汾河水环境保护提出了更高的要求。 |
| 关键词: 汾河景区 微囊藻 分子多样性 毒素 |
| DOI：10.18307/2011.0403 |
| 分类号: |
| 基金项目:中国科学院项目(082303-1-501)资助 |
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| Molecular diversity and microcystin production of Microcystis in Fenhe River of Taiyuan |
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WANG Jie1,2, XIE Shulian1, WANG Zhongjie2, XU Yao2, LI Renhui2
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1.School of Life Science, Shanxi University, Taiyuan 030006, P.R.China;2.Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, P.R.China
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| Abstract: |
| To study the molecular diversity of Microcystis in Fenhe River of Taiyuan,phylogenetic analyses based on ITS、 PC-IGS and gyrB genes were performed from 7 isolated strains of Microcystis.These seven Microcystis strains formed distinct lineages,sug-gesting high diversity of Microcystis.ELISA and whole-cell PCR targeting microcystin synthetase gene (mcyA) were used to deter-mine microcystin production.Results showed that six of the seven strains contain mcyA gene and the microcystin concentrations of the six Microcystis strains ranged from 0.003mg/g to 0.043mg/g.This is the first report of toxic Microcystis in Fenhe River,and it is urgent that more critical measures should be taken for the protection of Fenhe River. |
| Key words: Fenhe River Microcystis molecular diversity microcystin |
