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引用本文:王健,周天艺,高文程,浦海清,杨柳燕.低氧化还原电位对铜绿微囊藻(Microcystis aeruginosa)生长的抑制及恢复的影响.湖泊科学,2012,24(4):528-534. DOI:10.18307/2012.0404
WANG Jian,ZHOU Tianyi,GAO Wencheng,PU Haiqing,YANG Liuyan.Growth inhibition and recovery from low oxidation reduction potential to Microcystis aeruginosa. J. Lake Sci.2012,24(4):528-534. DOI:10.18307/2012.0404
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低氧化还原电位对铜绿微囊藻(Microcystis aeruginosa)生长的抑制及恢复的影响
王健1, 周天艺1, 高文程1, 浦海清1, 杨柳燕1,2
1.南京大学环境学院, 南京 210046;2.污染控制与资源化研究国家重点实验室, 南京 210046
摘要:
在实验室光照黑暗交替培养下,用半胱氨酸下调黑暗起始时的氧化还原电位(ORP),研究低电位对铜绿微囊藻(Microcystis aeruginosa FACHB 469)生长的影响,并探索低电位胁迫消除后铜绿微囊藻生长的恢复潜力.结果表明,在-50~-100 mV范围内水体ORP越低,其对铜绿微囊藻生长的抑制作用越强.低ORP可抑制铜绿微囊藻对磷的吸收,且这种抑制程度与电位变化无关.-50~-75 mV的电位对铜绿微囊藻SOD酶活性有促进作用,而-90~-100 mV电位明显抑制SOD酶活.当低ORP胁迫消除后,铜绿微囊藻能够恢复至最初的生长状态,同时伴随含磷量的提高和SOD酶活的恢复,-50~-75 mV电位胁迫对藻消除胁迫后的生长更有促进作用.因此,低ORP对铜绿微囊藻的生长会产生不利影响,但是该影响是可逆的.
关键词:  铜绿微囊藻  氧化还原电位  生长抑制  恢复    SOD酶  叶绿素a
DOI:10.18307/2012.0404
分类号:
基金项目:国家重点基础研究发展计划"973"项目(2008CB418102);环保公益性行业科研专项项目(200809145);南京大学大学生创新训练计划项目联合资助
Growth inhibition and recovery from low oxidation reduction potential to Microcystis aeruginosa
WANG Jian1, ZHOU Tianyi1, GAO Wencheng1, PU Haiqing1, YANG Liuyan1,2
1.School of the Environment, Nanjing University, Nanjing 210046, P. R. China;2.State Key Laboratory of Pollution Control and Resource Reuse, Nanjing 210046, P. R. China
Abstract:
In this experiment, cysteine was used as an Oxidation Reduction Potential (ORP) adjustment to explore the responses of Microcystis aeruginosa (FACHB 469) under different ORPs'stimulation. A recovery experiment was also designed with M. aeruginosa separated from the former experiment and cultured in fresh medium. Results showed a negative effect of low ORP stimulation on the growth of M. aeruginosa. Within the range of -50 to -100 mV, inhibition effect was intensified with the ORP's decline. The uptake rate of phosphorus decreased under low ORP's stress but had no relationship with stress strength. A proper low ORP' s stimulation (e. g. -50 and -75 mV) could intensify the SOD enzyme activity of M. aeruginosa, while over low (e. g. -90 and -100 mV) had negative effects. M. aeruginosa could recover its growing ability after removing the lower ORP's stimulation. The uptake rate of phosphorus and the SOD enzyme activity returned to normal as well. Moreover, the enhancement of growth ability was observed by a low ORP's stimulation (e. g. -50 and -75 mV). Thus, the low ORP's stimulation had negative effect on the growth of M. aeruginosa, however the process can be reversible.
Key words:  Microcystis aeruginosa  oxidation reduction potential  growth limit  recovery  phosphorus  SOD enzyme  chlorophyll-a
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