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引用本文:叶琳琳,史小丽,吴晓东,于洋,张民,孔繁翔.太湖浮游植物细胞裂解速率的酯酶活性法初步研究.湖泊科学,2012,24(5):712-716. DOI:10.18307/2012.0511
YE Linlin,SHI Xiaoli,WU Xiaodong,YU Yang,ZHANG Min,KONG Fanxiang.Assessing phytoplankton cell lysis rate in Lake Taihu by esterase assay. J. Lake Sci.2012,24(5):712-716. DOI:10.18307/2012.0511
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太湖浮游植物细胞裂解速率的酯酶活性法初步研究
叶琳琳1,2, 史小丽2, 吴晓东3, 于洋2, 张民2, 孔繁翔2
1.南通大学地理科学学院, 南通 226019;2.中国科学院南京地理与湖泊研究所湖泊与环境国家重点实验室, 南京 210008;3.中国科学院寒区旱区环境与工程研究所, 兰州 730000
摘要:
本研究从2009年8月至2010年10月,每月采集太湖3个不同富营养化湖区水样,运用酯酶活性法,测定了颗粒态酯酶、溶解性酯酶活性以及酯酶衰变周期,估算了太湖浮游植物细胞裂解速率.研究结果表明,太湖颗粒态酯酶活性为0.58~35.15 nmol FDA/(L.h),溶解性酯酶活性为0.55~7.59 nmol FDA/(L.h),酯酶衰变周期为7~75 h,细胞裂解速率为0.02~0.77 d-1,三个采样点细胞裂解速率没有显著差异.颗粒态酯酶活性与叶绿素a浓度之间具有显著的线性关系,说明运用酯酶活性法估算太湖浮游植物细胞裂解速率是可行.此外,叶绿素a浓度与温度变化趋势基本一致,梅梁湾和湖心叶绿素a浓度具有显著差异.贡湖湾叶绿素a浓度与细胞裂解速率之间具有显著的反比例关系,说明细胞裂解速率也是影响太湖藻类生物量的重要因素.
关键词:  酯酶活性  太湖  细胞裂解速率  浮游植物
DOI:10.18307/2012.0511
分类号:
基金项目:国家重点基础研究发展计划"973"项目(2008CB418005,2008CB418000);国家自然科学基金项目(41001032,40901268)联合资助
Assessing phytoplankton cell lysis rate in Lake Taihu by esterase assay
YE Linlin1,2, SHI Xiaoli2, WU Xiaodong3, YU Yang2, ZHANG Min2, KONG Fanxiang2
1.Geographical Sciences College, Nantong University, Nantong 226019, P. R. China;2.State Key Laboratory of Lake Science and Environment, Nanjing Institute of Geography and Limnology, Chinese Academy of Sciences, Nanjing 210008, P. R. China;3.Cold and Arid Regions Environmental and Engineering Research Institute, Chinese Academy of Sciences, Lanzhou 730000, P. R. China
Abstract:
Phytoplankton lysis rate in three different eutrophic regions of Lake Taihu was measured by the activities of particle and dissolved esterase, as well as the decay rate of the latter, from August 2009 to October 2010. The present results showed that the particulate, dissolved esterase activity, half life time of dissolved esterase, and cell lysis rates ranged from 0.58-35.15 nmol FDA/(L·h), 0.55-7.59 nmol FDA/(L·h), 7-75 h, and 0.02-0.77 d-1, respectively. No significant differences were found in the cell lysis rates among the three sampling sites. The significant linear relationship between Chl.a concentration and particulate esterase activities suggests that it is feasible to assess phytoplankton cell lysis rate in Lake Taihu by the esterase assay. Furthermore, Chl.a concentration showed the same pattern with water temperature, a significant difference was observed in Chl.a concentration between Meiliang Bay and lake centre. The strong, negative correlation between Chl.a concentration and lysis rate in Gonghu Bay suggests that phytoplankton biomass was affected by the lysis rate in Lake Taihu.
Key words:  Esterase assay  Lake Taihu  cell lysis rate  phytoplankton
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