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引用本文:陈霖,姜岩,汪鹏合,施国新,乔绪强,田秀丽.镍胁迫对菹草(Potamoge toncrispus L.)活性氧及脯氨酸代谢的影响.湖泊科学,2013,25(1):131-137. DOI:10.18307/2013.0117
CHEN Lin,JIANG Yan,WANG Penghe,SHI Guoxin,QIAO Xuqiang,TIAN Xiuli.Effect of nickel stress on reactive oxygen species and proline metabolism of Potamoge toncrispus L.. J. Lake Sci.2013,25(1):131-137. DOI:10.18307/2013.0117
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镍胁迫对菹草(Potamoge toncrispus L.)活性氧及脯氨酸代谢的影响
陈霖, 姜岩, 汪鹏合, 施国新, 乔绪强, 田秀丽
南京师范大学生命科学学院, 江苏省生物多样性与生物技术重点实验室, 南京 210046
摘要:
本文以组织培养技术培养的菹草无菌苗为实验材料,研究了不同浓度(0、0.05、0.10、0.15、0.20 mmol/L)Ni2+胁迫对菹草过氧化氢(H2O2)、超氧阴离子(O2.-)和丙二醛(MDA)的含量、抗氧化系统、脯氨酸代谢途径的影响.结果显示:(1)Ni2+胁迫影响了活性氧水平,主要表现为H2O2和O2.-的大量积累;MDA含量也随着Ni2+浓度的升高呈上升趋势;(2)随着Ni2+浓度的升高,超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)活性及还原型谷胱甘肽(GSH)、非蛋白巯基(NP-SH)含量均表现为先升后降趋势;抗坏血酸(AsA)含量变化不明显;植物络合素(PCs)则表现为上升趋势;(3)脯氨酸代谢关键酶在Ni2+胁迫下表现为:鸟氨酸转氨酶(OAT)活性在0~0.10 mmol/L Ni2+胁迫下高于对照组;吡咯啉-5-羧酸合成酶(P5CS)活性在Ni2+大于0.10 mmol/L时,显著增加;脯氨酸脱氢酶(ProDH)活性无显著变化;脯氨酸(Pro)含量则均显著高于对照组.以上结果表明,Ni2+胁迫提高了活性氧的水平,加重了膜脂过氧化的程度,干扰了抗氧化系统的内在平衡;此外,低浓度Ni2+胁迫下菹草脯氨酸积量主要依赖于鸟氨酸途径,而高浓度下则主要依靠谷氨酸途径.
关键词:  菹草  Ni2+胁迫  活性氧  脯氨酸代谢
DOI:10.18307/2013.0117
分类号:
基金项目:江苏高校优势学科建设工程项目(164320H106)资助
Effect of nickel stress on reactive oxygen species and proline metabolism of Potamoge toncrispus L.
CHEN Lin, JIANG Yan, WANG Penghe, SHI Guoxin, QIAO Xuqiang, TIAN Xiuli
Jiangsu Key Lab of Biodiversity and Biotechnology, College of Life Science, Nanjing Normal University, Nanjing 210046, P.R.China
Abstract:
Sterile seedlings of Potamogeton crispus were cultured by tissue culture technique,and used as experimental materials.In this study,different concentrations of Ni2+(0,0.05,0.10,0.15 and 0.20 mmol/ L) on contents of hydrogen peroxide (H2O2),superoxide anion (O2.-) and malondialdehyde (MDA),antioxidant defense system and proline metabolism were investigated.The results indicated that (1) Ni2+stress triggered the generation of reactive oxygen species (ROS) as evidenced by a substantial accumulation of H2O2 and O2.-,accompanied by the increased MDA content with the increasing of Ni2+concentration;(2) Activities of superoxide dismutase (SOD),peroxides (POD) and catalase (CAT),as well as contents of reduced glutathione (GSH) and non-protein thiols (NP-SH) increased at first and then decreased,while no outstanding changes were observed in ascorbate (AsA) content,and level of phytochelatins (PCs) increased significantly;(3) Analysis of enzyme activities showed that the accumulation of proline induced by Ni2+stress is due to the increases of ornithine-δ-aminotransferase (OAT) at 0.05-0.10 mmol/L and pyrroline-5-carboxylate synthetase (P5CS) activity at 0.15-0.20 mmol/ L,respectively,but there was not a statistically significant difference in ProDH activity.Our results demonstrated that Ni2+induced the enhancement of reactive oxygen species level,aggravated lipid peroxidation and interfere with intrinsic balance of antioxidant defense system.In addition,proline accumulation is mediated by increased biosynthesis from ornithine at low concentrations (0.05 and 0.10 mmol/L),while glutamate pathway,instead of the ornithine pathway,plays a vital role when exposed to higher concentrations of Ni2+.
Key words:  Potamogeton crispus L.  Ni2+stress  reactive oxygen species  proline metabolism
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