| 引用本文: | 谷依露,高小峰,李仁辉.水华微囊藻(Microcystis flos-aquae)生长周期中腐殖酸的释放特性.湖泊科学,2015,27(4):623-628. DOI:10.18307/2015.0409 |
| GU Yilu,GAO Xiaofeng,LI Renhui.Characterization of humic acid release during the growth of Microcystis flos-aquae. J. Lake Sci.2015,27(4):623-628. DOI:10.18307/2015.0409 |
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| 摘要: |
| 由于严重的水体富营养化,太湖长期暴发蓝藻水华,这些蓝藻在代谢及消亡过程中大量释放包括腐植酸在内的有机物,不仅影响供水水质,并且进一步加重了水华程度.以太湖水华优势藻种——水华微囊藻(Microcystis flos-aquae T34)为研究对象,对藻株生长周期中的藻细胞、浮游细菌进行计数,并使用TOC仪与三维荧光光谱对培养液中提取的腐殖酸进行定量与定性分析,探究水华微囊藻在生长过程中藻细胞密度、浮游细菌密度与腐殖酸浓度的变化规律.结果表明,培养液中腐殖酸浓度的变化趋势与细菌数量变化趋势一致,但与细菌数量曲线拐点相比,腐殖酸浓度曲线拐点出现两周的延迟.腐殖酸产量在藻细胞对数期较低,当水华微囊藻进入稳定期与衰亡期后,腐殖酸大量产生,其浓度迅速增加,最高可达到28.6 mg/L.根据三维荧光光谱分析,水华微囊藻所产生的腐殖酸特征峰出现在(235~245 nm)/(380~425 nm),属于类富里酸荧光峰.本研究初步探究蓝藻与水体腐殖酸之间的关系,为水体腐殖酸来源的研究提供了新的思路. |
| 关键词: 水华微囊藻 腐殖酸 三维荧光光谱 |
| DOI：10.18307/2015.0409 |
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| 基金项目:国家自然科学基金国际(地区)合作交流项目(41130743)资助. |
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| Characterization of humic acid release during the growth of Microcystis flos-aquae |
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GU Yilu1,2, GAO Xiaofeng3, LI Renhui1
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1.Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, P.R.China;2.University of Chinese Academy of Sciences, Beijing 100049, P.R.China;3.State Key Laboratory of Pollution Control and Resource Reuse, College of Environmental Sciences and Engineering, Tongji University, Shanghai 200092, P.R.China
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| Abstract: |
| Microcystis flos-aquae T34 strain, isolated from the dominant species of a water bloom in Lake Taihu, was cultivated to investigate its growth, along with bacterial growth in the culture and the release of humic acid. It was shown that the changing tendency of humic acid was similar to that of the bacteria, but the turning point of the former had two weeks' delay compared with that of the latter. During the logarithmic phase of Microcystis flos-aqaue growth, the release of humic acid was relatively low, however the concentration of humic acid in the culture solution increased rapidly up to 28.6 mg/L. Based on the three dimensional EEM fluorescence spectrum analyses, the characteristic peak of humic acid from Microcystis flos-aquae T34 appeared as (235-245 nm)/(380-425 nm), which is the typical fluorescence peak of a kind of fulvic acid. The result about the association between cyanobacteria and humic acid in this study will provide a new clue for the study of humic acid in aquatic ecosystems. |
| Key words: Microcystis flos-aquae humic acid 3D-EEM fluorescence spectrum |
