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引用本文:徐宇,钟立强,李潇轩,朱锡和,王崇华,史杨白.5个湖泊河川沙塘鳢(Odontobutis potamophila)种群线粒体细胞色素b基因的遗传变异分析.湖泊科学,2015,27(4):693-699. DOI:10.18307/2015.0418
XU Yu,ZHONG Liqiang,LI Xiaoxuan,ZHU Xihe,WANG Chonghua,SHI Yangbai.Genetic variation analysis of Odontobutis potamophila from five lakes based on Mitochondrial DNA cyt b. J. Lake Sci.2015,27(4):693-699. DOI:10.18307/2015.0418
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5个湖泊河川沙塘鳢(Odontobutis potamophila)种群线粒体细胞色素b基因的遗传变异分析
徐宇1, 钟立强1,2, 李潇轩1, 朱锡和1, 王崇华1, 史杨白1
1.江苏省淡水水产研究所, 南京 210017;2.农业部淡水渔业健康养殖重点实验室, 浙江省淡水水产研究所, 湖州 313001
摘要:
通过对5个湖泊的河川沙塘鳢种群的线粒体DNA细胞色素b基因进行PCR扩增、测序,获得1141bp的序列全长.序列分析显示,cyt b基因序列中A+T含量(55.8%)略高于G+C含量(44.2%),共检测到806个多态位点,115个样本得到87个单倍型,平均单倍型多样性为0.969±0.012,核苷酸多样性为0.20081±0.00742,遗传多样性表现高度多样性.太湖种群与大纵湖种群间的遗传距离最近,为0.137,巢湖种群和大纵湖种群之间遗传距离最远,为0.424.分子方差分析表明,群体间遗传分化系数Fst为0.531,变异来自群体内及群体间.cyt b基因序列构建的UPGMA系统进化树显示,5个种群分化成不同的分支系谱,种群间存在的基因交流较少.
关键词:  河川沙塘鳢  线粒体DNA  细胞色素b基因  遗传变异
DOI:10.18307/2015.0418
分类号:
基金项目:江苏省科技支撑计划项目(BE2011331)、江苏省水产三新工程重大项目(D2013-1)和农业部淡水渔业健康养殖重点实验室开放课题(ZJK2013132)联合资助.
Genetic variation analysis of Odontobutis potamophila from five lakes based on Mitochondrial DNA cyt b
XU Yu1, ZHONG Liqiang1,2, LI Xiaoxuan1, ZHU Xihe1, WANG Chonghua1, SHI Yangbai1
1.Freshwater Fisheries Research Institute of Jiangsu Province, Nanjing 210017, P.R.China;2.Key Laboratory of Fish Health and Nutrition of Zhejiang Province, Zhejiang Institute of Freshwater Fisheries, Huzhou 313001, P.R.China
Abstract:
The mtDNA cyt b gene of Odontobutis potamophila from five lakes was amplified and sequenced. Result showed that the average content of A+T(55.8%) was a little higher than that of G+C(44.2%),in the aligned sequences of 1141bp. 806 variable sites were detected and 87 haplotypes were recovered. The haplotypes diversity and nucleotide diversity were 0.969±0.012 and 0.20081±0.00742, respectively. The genetic distance between Lake Taihu population and Lake Dazonghu population displayed the lowest, up to 0.137, while that between Lake Chaohu population and Lake Dazonghu population was the highest to 0.424.The fixation indices of analysis of molecular variance among populations was 0.531, The phylogenetic tree of cyt b built with MEGA 4.1 showed that five populations were clustered into clades based on genetic distance. There was little general flow between O. potamophila populations from five lakes.
Key words:  Odontobutis potamophila  Mitochondrial DNA  cyt b  genetic variation
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