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引用本文:戴江玉,高光,吴时强,吴修锋,周杰,薛万云,杨倩倩,陈丹.水体细菌碱性磷酸酶及其编码基因研究进展.湖泊科学,2016,28(6):1153-1166. DOI:10.18307/2016.0601
DAI Jiangyu,GAO Guang,WU Shiqiang,WU Xiufeng,ZHOU Jie,XUE Wanyun,YANG Qianqian,CHEN Dan.Bacterial alkaline phosphatases and affiliated encoding genes in natural waters: A review. J. Lake Sci.2016,28(6):1153-1166. DOI:10.18307/2016.0601
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水体细菌碱性磷酸酶及其编码基因研究进展
戴江玉1, 高光2, 吴时强1, 吴修锋1, 周杰1, 薛万云1, 杨倩倩1, 陈丹3
1.南京水利科学研究院水文水资源与水利工程科学国家重点实验室, 南京 210029;2.中国科学院南京地理与湖泊研究所湖泊与环境国家重点实验室, 南京 210008;3.环境保护部南京环境科学研究所南京国环科技股份有限公司, 南京 210042
摘要:
水体溶解性反应磷是浮游植物可直接利用的主要磷营养形式,细菌碱性磷酸酶催化的有机磷矿化过程则是维持溶解性反应磷供给的重要途径.通过总结细菌碱性磷酸酶的种类与分布特征,发现主要包括PhoA、PhoX和PhoD 3种类型细菌碱性磷酸酶,其中超过50%的PhoX分布于细菌细胞的外部环境,对溶解性反应磷的循环再生尤为重要.综述水体产碱性磷酸酶细菌群落组成的研究进展,以及影响编码基因表达的环境因素,对比分析产碱性磷酸酶细菌群落鉴定方法的特点及发展趋势.细菌碱性磷酸酶编码基因的研究多关注寡营养海洋生态系统,对于同样存在磷限制问题的富营养化湖泊生态系统鲜有涉及,而构建针对典型富营养化湖泊生态系统的细菌宏基因组数据库,则可为建立淡水生态系统细菌碱性磷酸酶及其编码基因的研究方法奠定基础,有助于认识湖泊水华频发的微生物学驱动机制.
关键词:    细菌  碱性磷酸酶  编码基因  湖泊
DOI:10.18307/2016.0601
分类号:
基金项目:国家水体污染控制与治理科技重大专项(2012ZX07506-003-04)、国家自然科学基金项目(51309156,51479120,51479121)、江苏省自然科学基金项目(BK20141075)、水利部公益性行业科研专项(201501015-01)和南京水利科学研究院院基金项目(Y116006)联合资助.
Bacterial alkaline phosphatases and affiliated encoding genes in natural waters: A review
DAI Jiangyu1, GAO Guang2, WU Shiqiang1, WU Xiufeng1, ZHOU Jie1, XUE Wanyun1, YANG Qianqian1, CHEN Dan3
1.State Key Laboratory of Hydrology-Water Resources and Hydraulic Engineering, Nanjing Hydraulic Research Institute, Nanjing 210029, P. R. China;2.State Key Laboratory of Lake Science and Environment, Nanjing Institute of Geography and Limnology, Chinese Academy of Sciences, Nanjing 210008, P. R. China;3.Nanjing Guohuan Science and Technology Co., Ltd of Nanjing Institute of Environmental Sciences, MEP, Nanjing 210042, P. R. China
Abstract:
Soluble reactive phosphorus is the main form of phosphorus directly available for phytoplankton in natural waters, and the organophosphate mineralization catalyzed by bacterial alkaline phosphatases maintains the supply of soluble reactive phosphorus. We summarized the species and subcellular location of bacterial alkaline phosphatases and found that PhoA, PhoX and PhoD were the main phosphatase species. Over 50% of PhoX were distributed outside of bacterial cells, which is particularly important for the regeneration of soluble reactive phosphorus in natural waters. The research progress in communities of alkaline phosphatase encoding bacteria and the environmental factors influencing the expression of alkaline phosphatase encoding genes were also reviewed. We compared and analyzed the characteristics and developing trend of identification methods towards bacterial communities generating alkaline phosphatases. Most of studies about bacterial alkaline phosphatase-encoding genes were focused on oligotrophic marine ecosystems, and little was on the lake ecosystems which are also facing the phosphorus-limiting problem. Further researches should construct the bacterial metagenomic databases for certain eutrophic lakes to provide basis for build the methodology for bacterial alkaline phosphatases and affiliated encoding genes in freshwater ecosystems, which are beneficial to understand the microbial mechanisms driving frequent cyanobacterial blooms in lakes.
Key words:  Phosphorus  bacteria  alkaline phosphatase  encoding gene  lake
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