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引用本文:王晓莹,张明珍,严攀,陈迪松,王亚芬,周巧红,吴振斌,徐栋.美人蕉(Canna indica)内生细菌促生能力及其强化水体的净化作用.湖泊科学,2019,31(6):1582-1591. DOI:10.18307/2019.0605
WANG Xiaoying,ZHANG Mingzhen,YAN Pan,CHEN Disong,WANG Yafen,ZHOU Qiaohong,WU Zhenbin,XU Dong.Growth promoting ability and enhanced effects on water purification of endophytic bacteria from Canna indica. J. Lake Sci.2019,31(6):1582-1591. DOI:10.18307/2019.0605
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美人蕉(Canna indica)内生细菌促生能力及其强化水体的净化作用
王晓莹1,2, 张明珍1,2, 严攀1,2, 陈迪松1, 王亚芬3, 周巧红1, 吴振斌1, 徐栋1
1.中国科学院水生生物研究所淡水生态与生物技术国家重点实验室, 武汉 430072;2.中国科学院大学, 北京 100049;3.中国地质大学湖北省水环境污染系统控制和治理工程技术研究中心, 武汉 430074
摘要:
从美人蕉(Canna indica)植物体内分离内生细菌.通过测定内生细菌产吲哚乙酸(IAA)、铁载体、固氮、溶磷能力筛选出3株内生细菌C01、C16、C20.本试验的目的为评价接种内生细菌能否促进美人蕉种子萌发和幼苗生长,并通过小型浮床水质净化试验,评价接种内生细菌后的美人蕉对水体中营养物质的去除效果.结果表明:C01、C16、C20促进了美人蕉种子的萌发、生长,C16试验组萌发率为83.3%,显著高于对照组31.1%;C16显著提高了植株的株高和根长,分别比对照组高出23.33%、40.44%.水质净化实验阶段,C16试验组总磷、总氮的去除率分别达到88.4%、96.9%,分别比对照组高出26.4%、15.6%.最终,C01、C16试验组生物量、根长都显著高于对照组.C16试验组植株体内可溶性糖含量、可溶性蛋白显著高于对照组317.3%、97.8%.经鉴定,C01为荧光假单胞菌(Pseudomonas fluorescens),C16为肠杆菌(Enterobacter sp.),C20为欧文氏菌(Erwinia sp.).
关键词:  美人蕉  内生细菌  促生能力  水体净化
DOI:10.18307/2019.0605
分类号:
基金项目:国家水体污染控制与治理科技重大专项(2017ZX07602002-004)和中国科学院科技服务网络计划(KFJ-STS-ZDTP-038)联合资助.
Growth promoting ability and enhanced effects on water purification of endophytic bacteria from Canna indica
WANG Xiaoying1,2, ZHANG Mingzhen1,2, YAN Pan1,2, CHEN Disong1, WANG Yafen3, ZHOU Qiaohong1, WU Zhenbin1, XU Dong1
1.State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, P. R. China;2.Univerisity of Chinese Academy of Sciences, Beijing 100049, P. R. China;3.Hubei Provincial Engineering Research Center of Systematic Water Pollution Control, China University of Geosciences, Wuhan 430074, P. R. China
Abstract:
Endophytic bacteria were isolated from Canna indica, and three strains C01, C16 and C20 were screened out regard to their abilities of producing indoleacetic acid (IAA) and siderophore, nitrogen fixation, as well as phosphate solubilization. The aim of this study is to evaluate the influence of inoculating of endophytic bacteria on seed germination and seedling growth of C. indica, and to evaluate the removal rates of nutrients in water body by C. indica inoculated with endophytic bacteria in the pilot scale floating bed treatment system. The results showed that C01, C16 and C20 promoted the germination of seed and growth of plants. The germination rate of C16 treatment was 83.3%, significantly higher than that of the control, which was 31.1%. During the water purification experimental stage, the removal rates of total phosphorus and total nitrogen in the C16 treatment were 88.4% and 96.9%, respectively, which were 26.4% and 15.6% higher than the control. At the end of the experiment, the biomass and root length of the C01 and C16 treatment were significantly higher than the control, the soluble sugar content and soluble protein content in the C16 treatment were 317.3% and 97.8% higher than those in the control respectively. C01, C16 and C20 were proved to be Pseudomoanas fluorescens, Enterobacter sp., Erwinia sp. based on 16S rRNA sequence analysis.
Key words:  Canna indica  endophytic bacteria  growth promoting ability  water purification
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