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引用本文:杨习文,刘熠,薛向平,王邢艳,文红,王小豪,徐东坡,周彦锋,方弟安.基于微卫星标记的长江江苏段鲢(Hypophthalmichthys molitrix)增殖放流资源贡献率的评估.湖泊科学,2020,32(4):1154-1164. DOI:10.18307/2020.0422
YANG Xiwen,LIU Yi,XUE Xiangping,WANG Xingyan,WEN Hong,WANG Xiaohao,XU Dongpo,ZHOU Yanfeng,FANG Di'an.Resource contribution rate assessment of stock enhancement of silver carp, Hypophthalmichthy smolitrix in Jiangsu section of the Yangtze River based on microsatellite markers. J. Lake Sci.2020,32(4):1154-1164. DOI:10.18307/2020.0422
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基于微卫星标记的长江江苏段鲢(Hypophthalmichthys molitrix)增殖放流资源贡献率的评估
杨习文1,2, 刘熠1,2, 薛向平1, 王邢艳2,3, 文红2,3, 王小豪2,3, 徐东坡1,2, 周彦锋1,2, 方弟安1,2
1.上海海洋大学水产科学国家级实验教学示范中心, 上海 201306;2.中国水产科学研究院淡水渔业研究中心农业农村部淡水渔业与种质资源利用重点实验室, 无锡 214081;3.南京农业大学无锡渔业学院, 无锡 214081
摘要:
近年来,长江下游进行了大规模鲢(Hypophthalmichthys molitrix)增殖放流活动,而增殖放流对鲢资源的贡献如何,成为亟待探究的科学问题.本研究基于已开发的11对微卫星引物,标记8个主要原良种场(为江苏省各地政府放流工作提供苗种的单位)鲢繁殖亲本群体,利用微卫星亲子鉴定技术,评估2016-2017年长江江苏段鲢增殖放流对鲢在江群体的资源贡献率.研究结果显示,621尾繁殖亲本群体和475尾长江捕捞鲢群体共检测出191种等位基因,每个座位的等位基因种类数、观测杂合度、期望杂合度范围分别为9~36、0.536~0.793、0.597~0.941;对应平均值分别为17.36、0.656、0.794.11个座位均表现出高度的多态性,每个座位的多态信息含量范围为0.560~0.938,平均值为0.771,证明选用的11对微卫星引物可作为亲子鉴定的有效工具.亲子鉴定结果表明,在亲本性别未知的情况下,非亲排除率(NEP)范围为2.4%~42.4%,累积非亲权排除率(CEP)在99.99%以上;475尾长江鲢群体中,鉴定出39尾鲢个体来自于原良种场鲢亲本群体所繁殖的后代,即回捕率为8.21%.综合分析可见,2016-2017年,长江江苏段增殖放流对鲢资源量有较好的补充作用.
关键词:    增殖放流  微卫星标记  亲子鉴定  资源贡献率  长江江苏段
DOI:10.18307/2020.0422
分类号:
基金项目:国家重点研发计划项目(2018YFD0900903)和农业部渔业种质资源保护项目(201801065)联合资助.
Resource contribution rate assessment of stock enhancement of silver carp, Hypophthalmichthy smolitrix in Jiangsu section of the Yangtze River based on microsatellite markers
YANG Xiwen1,2, LIU Yi1,2, XUE Xiangping1, WANG Xingyan2,3, WEN Hong2,3, WANG Xiaohao2,3, XU Dongpo1,2, ZHOU Yanfeng1,2, FANG Di'an1,2
1.National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, P. R. China;2.Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, P. R. China;3.Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, P. R. China
Abstract:
In recent years, the large-scale stock enhancement of silver carp, Hypophthalmichthys molitrix had been implemented in the lower reaches of the Yangtze River. It was urgent to study how much resource contribution the stock enhancement of silver carp produced. This study assessed resource contribution rate of stock enhancement of silver carp in Jiangsu section of the Yangtze River from the year 2016-2017, using microsatellite parentage testing technique. Before releasing fries, brood stocks of eight hatcheries, used for breeding, were marked with eleven pairs of ever developed microsatellite primers. These eight hatcheries also were the major institutions that supplied silver carp fries to the releasing work of governments around Jiangsu Province. The results showed that 191 types of alleles were detected between 621 brood stocks hatcheries and 475 captured silver carp in the Yangtze River. The number of different alleles (NA), the observed heterozygosity (Ho) and the expected heterozygosity (He) were 9-36, 0.536-0.793, 0.597-0.941, respectively, and their corresponding mean values were 17.36, 0.656 and 0.794. All of eleven microsatellite loci were high polymorphism, and the polymorphic information content (PIC) per locus ranged from 0.560 to 0.938 with the mean value of 0.771, which proved 11 pairs of microsatellite primers as an effective tool for parentage identification. Result of parentage testing showed that the non-exclusion probability (NEP) was from 2.4% to 42.4% at the case of parent's unknown gender, and the combined non-exclusion probability (CEP) was over 99.99%. Thirty-nine individuals from 475 silver carps of the Yangtze River were identified as the hatchery-reared offspring, i.e., the recapture rate 8.21%. Through comprehensive analysis, it was concluded that stock enhancement had produced a good supplement effect on silver carp resource from 2016 to 2017.
Key words:  Silver carp(Hypophthalmichthys molitrix)  stock enhancement  microsatellite markers  parentage testing  resource contribution rate  Jangsu section of the Yangtze River
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