| 引用本文: | 王丹丹,黄跃飞,杨海娇.青藏高原东北部湖泊细菌群落结构特征季节性差异及驱动机制.湖泊科学,2023,35(1):267-278. DOI:10.18307/2023.0121 |
| Wang Dandan,Huang Yuefei,Yang Haijiao.Seasonal differences of lake bacterial community structures and their driving mechanisms in the northeastern of the Qinghai-Tibet Plateau. J. Lake Sci.2023,35(1):267-278. DOI:10.18307/2023.0121 |
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| 青藏高原东北部湖泊细菌群落结构特征季节性差异及驱动机制 |
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王丹丹1, 黄跃飞1,2,3,4, 杨海娇1,3,4
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1.青海大学, 水利电力学院, 西宁 810016;2.青海大学, 省部共建三江源生态与高原农牧业国家重点实验室, 西宁 810016;3.青海大学, 黄河上游生态保护与高质量发展实验室, 西宁 810016;4.青海大学, 水利部江河源区水生态治理与保护重点实验室, 西宁 810016
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| 摘要: |
| 微生物是湖泊生态系统重要的有机组分。了解不同时期青藏高原湖泊细菌群落特征、环境驱动因子及其群落构建过程的差异,对高原湖泊水生态管理具有重要的指导意义。分别于2020年6和12月采集青藏高原东北部5个湖泊水样,基于高通量测序技术、统计分析和模型分析,解析高原湖泊群在非冰封期和冰封期细菌群落结构、环境驱动因子和中性过程、确定性过程以及随机性过程在细菌群落构建过程的贡献。结果表明:(1)非冰封期湖泊细菌群落的Chao1丰度指数、Simpson和Shannon多样性指数低于冰封期,Spearman相关性分析结果表明非冰封期细菌群落的Chao1丰度指数和多样性指数与水温和海拔显著相关,而冰封期细菌群落的Chao1指数和多样性指数与总氮显著相关;(2)水体细菌群落的优势门均为变形菌门(Proteobacteria),非冰封期细菌中拟杆菌门(Bacteroidetes)、放线菌门(Actinobacteria)和厚壁菌门(Firmicutes)的平均相对丰度明显高于冰封期。另外,主坐标分析和相似性分析结果表明非冰封期和冰封期的细菌群落组成差异极显著;(3)冗余分析结果表明不同时期湖泊群细菌群落结构的驱动因子不同,非冰封期环境因子影响的大小顺序为:总氮>纬度>总溶解固体>经度>溶解有机碳,冰封期为:海拔>溶解氧>溶解有机碳>pH;(4)Sloan中性模型计算结果表明冰封期高原湖泊细菌群落比非冰封期更易受到中性过程的驱动,并且冰封期细菌群落的迁移率也大于非冰封期(冰封期:0.289;非冰封期:0.130),反映冰封期细菌菌群扩散能力较强。随机性的扩散限制过程是非冰封期(贡献率=54.74%)和冰封期(贡献率=55.56%)湖泊细菌群落的主要构建过程。本文解析了不同时期高原湖泊细菌群落特征及其驱动机制,为高原湖泊水资源保护和管理以及深入理解细菌群落构建过程提供了基础。 |
| 关键词: 青藏高原 湖泊 细菌群落结构 驱动因子 中性模型 零模型 |
| DOI：10.18307/2023.0121 |
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| 基金项目:青海省重点研发与转化计划项目(2019-SF-146)资助。 |
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| Seasonal differences of lake bacterial community structures and their driving mechanisms in the northeastern of the Qinghai-Tibet Plateau |
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Wang Dandan1, Huang Yuefei1,2,3,4, Yang Haijiao1,3,4
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1.School of Hydraulic and Electric Engineering, Qinghai University, Xining 810016, P. R. China;2.State Key laboratory of Plateau Ecology and Agriculture, Qinghai University, Xining 810016, P. R. China;3.Provincial Laboratory of Ecological Protection and High Quality Development in the Upper Yellow River, Qinghai University, Xining 810016, P. R. China;4.Key Laboratory of Water Ecological Remediation and Protection at Headwater Regions of Big Rivers, Ministry of Water Resources, Qinghai University, Xining 810016, P. R. China
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| Abstract: |
| Microorganisms are important organic components of lake ecosystems. Understanding the differences in bacterial community structures, environmental driving factors and their community assembly processes in different periods in the lakes on the Qinghai-Tibet Plateau (QTP) has significant guiding role for water ecological management of the plateau lakes. Water samples were collected from five lakes on the northeastern of the QTP in June and December in 2020 for investigating the differences of community structures, environmental driving factors as well as the contributions of neutral processes, deterministic processes, and stochastic processes of bacterial communities in the plateau lakes between non-freezing and freezing periods using the high throughput sequencing, statistical analysis, and the model analysis. Results show that: (1) The Chao 1 richness index, Simpson, and Shannon diversity indices of bacterial communities were lower in non-freezing periods than those in freezing periods. Spearman correlation analysis revealed that water temperature and altitude were the most important factors in influencing Chao 1 richness index and diversity indices in non-freezing periods, while total nitrogen had a significant relationship with bacterial Chao 1 richness index and diversity indices in freezing periods. (2) The predominant phylum was Proteobacteria both in non-freezing and freezing periods. High average relative abundances of Bacteroidetes, Actinobacteria and Firmicutes were observed in non-freezing periods. Additionally, the results of principal coordinate analysis (PCoA) and similarity analysis showed that there were significant differences in the bacterial community compositions in non-freezing and freezing periods. (3) Results of redundancy analysis indicated that driving factors of bacterial community compositions were also different between non-freezing and freezing periods, which were in the order of total nitrogen>latitude>total dissolved solid>longitude>dissolved organic carbon in non-freezing periods and altitude>dissolved oxygen>dissolved organic carbon>pH in freezing periods, respectively. (4) Variations of bacterial communities were controlled by neutral process which was stronger in freezing periods than that in non-freezing periods and a higher dispersal rate was found in freezing periods than that in non-freezing periods (freezing periods: 0.289; non-freezing periods: 0.130) which reflected that the dispersal ability of bacterial species was higher in freezing periods than that in non-freezing periods. Dispersal limitation of stochastic processes shown to be a dominating process in the assembly of the bacterial communities both in non-freezing (contribution=54.74%) and freezing (contribution=55.56%) periods. Our study has analyzed the characteristics and driving mechanisms of bacterial communities in the plateau lakes in two different periods and will provide guidance for protection and management of the water resources in the plateau and basis for understanding bacterial community assembly processes. |
| Key words: Qinghai-Tibet Plateau lake bacterial community structures driving factors neutral model null model |
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