| 引用本文: | 王丹丹,黄跃飞,杨海娇.青藏高原湖泊沉积物与水体细菌群落共发生网络和群落构建过程差异解析.湖泊科学,2023,35(3):959-971. DOI:10.18307/2023.0316 |
| Wang Dandan,Huang Yuefei,Yang Haijiao.Differences of bacterial community co-occurrence network and assembly processes between sediment and water in lakes on the Qinghai-Tibet Plateau. J. Lake Sci.2023,35(3):959-971. DOI:10.18307/2023.0316 |
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| 青藏高原湖泊沉积物与水体细菌群落共发生网络和群落构建过程差异解析 |
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王丹丹1, 黄跃飞1,2,3,4, 杨海娇1,3,4
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1.青海大学, 水利电力学院, 西宁 810016;2.青海大学, 省部共建三江源生态与高原农牧业国家重点实验室, 西宁 810016;3.青海大学, 黄河上游生态保护与高质量发展实验室, 西宁 810016;4.青海大学, 水利部江河源区水生态治理与保护重点实验室, 西宁 810016
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| 摘要: |
| 研究高原湖泊沉积物和水体细菌群落结构的差异对于水生态治理与保护具有重要的科学意义。本文选取青藏高原中东部共19个湖泊为研究对象，采集沉积物和水体样本，使用高通量测序技术和统计分析，解析湖泊沉积物和水体细菌群落结构、空间分布特征、共发生网络模式、群落构建过程的差异及其原因。研究结果表明沉积物细菌群落alpha多样性指数（Chao1指数、Shannon多样性指数、Faith's系统发育指数和Pielou均匀度指数）均显著高于水体细菌群落。沉积物和水体细菌的优势门均为Proteobacteria （相对丰度为45.33%和41.77%），而水体细菌群落结构具有较强的空间异质性。主坐标分析和相似性分析结果表明沉积物和水体中细菌群落结构差异极显著，海拔和纬度分别是青藏高原湖泊沉积物和水体细菌群落结构变化最显著的驱动因子。沉积物和水体细菌群落均具有明显的距离衰减模式，而沉积物细菌比水体细菌群落在空间上的周转率小。采样湖泊沉积物细菌群落共发生网络复杂度和稳定性高于水体细菌。沉积物和水体细菌群落的平均归一化随机率值分别为0.24和0.72，表明确定性过程在沉积物细菌群落构建的过程中起到了重要的作用，而水体细菌群落的主导构建过程为随机性过程。本文在高原湖泊沉积物和水体中细菌群落特征、空间分布模式、生态网络稳定性、群落构建过程及其差异方面的研究，为深入理解高原湖泊细菌多样性和群落结构形成机理提供了科学支撑。 |
| 关键词: 高原湖泊 细菌群落 空间分布 共发生网络 群落构建 |
| DOI：10.18307/2023.0316 |
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| 基金项目:青海省重点研发与转化计划项目（2019-SF-146）资助。 |
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| Differences of bacterial community co-occurrence network and assembly processes between sediment and water in lakes on the Qinghai-Tibet Plateau |
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Wang Dandan1, Huang Yuefei1,2,3,4, Yang Haijiao1,3,4
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1.School of Hydraulic and Electric Engineering, Qinghai University, Xining 810016, P. R. China;2.State Key Laboratory of Plateau Ecology and Agriculture, Qinghai University, Xining 810016, P. R. China;3.Provincial Laboratory of Ecological Protection and High Quality Development in the Upper Yellow River, Qinghai University, Xining 810016, P. R. China;4.Key Laboratory of Water Ecological Remediation and Protection at Headwater Regions of Big Rivers, Ministry of Water Resources, Qinghai University, Xining 810016, P. R. China
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| Abstract: |
| It is of great scientific significance to study feature differences of bacterial community structure between sediment and water in the plateau lakes for water ecological management and protection. We selected 19 objective lakes in the central and eastern of the Qinghai-Tibet Plateau, collected the samples of sediment and water, and analyzed differences of bacterial community structures, spatial distributions, co-occurrence network patterns, community assembly processes between sediment and water in lakes based on high-throughput sequencing and statistical analysis. The results showed that alpha diversity indices of bacterial community in sediment, including Chao1, Shannon diversity, Faith's phylogenetic diversity and Pielou's evenness indices, were significantly higher than those in water. Dominant phylum in both sediment and water was Proteobacteria with the relative abundances of 45.33% and 41.77%, respectively, while bacterial community structures in water presented stronger spatial heterogeneity than in sediment. The results of principal coordinate analysis and similarity analysis showed that there were significant differences in bacterial community composition between sediment and water. Altitude and Latitude were the most important environmental drivers in shaping bacterial community compositions in sediment and water. Both sediment and water bacterial community had distance-decay patterns, while bacterial spatial turnover rate in sediment was lower than that in water. The co-occurrence network complexity and stability of bacterial community in sediment were higher than those in water. The mean value of normalized stochasticity ratios in sediment and water bacterial were 0.24 and 0.72, indicating deterministic process played a key role in sediment bacterial community assembly processes, whereas water bacterial community assembly processes was predominant by stochastic process. These findings about bacterial community features, spatial patterns, stability of co-occurrence networks, assembly processes, and their differences of bacterial between lake sediment and water in the Qinghai-Tibet Plateau would provide the scientific support for in-depth understanding mechanisms of bacterial diversity and composition formation in plateau lakes. |
| Key words: Plateau lakes bacterial community spatial distribution co-occurrence network community assembly |
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